Xia Yun, Qian Xing-Feng, Chen Xin, Wang Bo, Wang Yong-Xia
School of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan, 650214, People's Republic of China.
Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, People's Republic of China.
Curr Microbiol. 2024 Dec 21;82(1):51. doi: 10.1007/s00284-024-04008-w.
A novel bacteria strain, designated YIM B02787, was isolated from rhizosphere soil of Ageratina adenophora, in Yunnan, southwest China. The strain was aerobic, Gram-stain-negative, rod-shaped and motile with one polar flagellum. Growth occurred at 4-45 °C (optimum, 20-30 °C) and pH 6.0-10.0 (optimum, 7.0-8.0), and in presence of 0-1% (w/v) NaCl. Phylogenetic analyses based on both 16S rRNA gene and genome sequences data revealed that strain YIM B02787 belongs to the genus Massilia, being closely related to Massilia phosphatilytica KCTC 52513 (98.93% similarity), M. putida KCTC 42761 (98.86%), and M. kyonggiensis JCM 19189 (98.78%). The DNA G+C content was 65.9%. The digital DNA-DNA hybridization and average nucleotide identity values between the isolate strain and aforementioned closely neighbors were low, at 35.8-48.9 and 88.5-92.5%, respectively. Strain YIM B02787 contained Q-8 as the ubiquinone and major fatty acids were summed feature 3 (Cω7c and/or Cω6c, 45.5%) and C (27.5%). The polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, two unidentified phospholipids, two unidentified aminophospholipids, and one unidentified polar lipid. On the basis of its phylogenetic, phenotypic and chemotaxonomic characteristics, strain YIM B02787represents a novel species of the genus Massilia, for which the name Massilia orientalis sp. nov. is proposed. The type strain is YIM B02787 (= NBRC 116628 = CGMCC 1.61539).
从中国西南部云南省紫茎泽兰根际土壤中分离出一株新型细菌菌株，命名为YIM B02787。该菌株为需氧菌，革兰氏染色阴性，杆状，具一根极生鞭毛，能运动。在4-45℃（最适温度为20-30℃）、pH值6.0-10.0（最适pH值为7.0-8.0）以及0-1%（w/v）NaCl存在的条件下可生长。基于16S rRNA基因和基因组序列数据的系统发育分析表明，菌株YIM B02787属于马赛菌属，与解磷马赛菌KCTC 52513（相似度98.93%）、恶臭马赛菌KCTC 42761（98.86%）以及京畿道马赛菌JCM 19189（98.78%）密切相关。DNA G+C含量为65.9%。该分离菌株与上述近缘菌株之间的数字DNA-DNA杂交值和平均核苷酸同一性值较低，分别为35.8-48.9%和88.5-92.5%。菌株YIM B02787以Q-8作为泛醌，主要脂肪酸为总和特征3（Cω7c和/或Cω6c，45.5%）和C（27.5%）。极性脂质谱由磷脂酰甘油、磷脂酰乙醇胺、二磷脂酰甘油、两种未鉴定的磷脂、两种未鉴定的氨基磷脂和一种未鉴定的极性脂质组成。基于其系统发育、表型和化学分类学特征，菌株YIM B02787代表马赛菌属的一个新物种，为此提出新名称东方马赛菌（Massilia orientalis sp. nov.）。模式菌株为YIM B02787（=NBRC 116628=CGMCC 1.61539）。
