Chu Shufang, Liu Deliang, Zhao Hengxia, Liu Ling, Li Juntong, Wang Gaoxiang, Liu Xuemei, Li Huilin
Department of Endocrinology, Shenzhen Traditional Chinese Medicine Hospital, No. 1, Futian District, Shenzhen, 518033, Guangdong, China.
Department of Endocrinology, Shenzhen Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Shenzhen, 518033, China.
Genes Genomics. 2025 Feb;47(2):263-273. doi: 10.1007/s13258-024-01607-6. Epub 2024 Dec 21.
Baihu Jia Renshen Decoction (BJRD) is used for diabetes mellitus (DM) management in clinics.
To elucidate the potential mechanism of BJRD in treating type 1 DM (T1DM).
T1DM models were established via intraperitoneal injection of streptozotocin (STZ). Rats were subsequently randomly divided into the normal control (NC), model (MOD), insulin (INS), INS + BJRD-medium dose (MID), and INS + BJRD-high dose (HIGH) groups. The rats' body weight was measured. Transcriptome sequencing was performed to detect differentially expressed genes (DEGs) in the muscle and adipose tissues. Quantitative real-time polymerase chain reaction was utilized to verify the DEG levels.
Body weights of MOD, INS, MID, and HIGH groups were significantly reduced as compared to those of NC group. Compared with NC group, MOD group showed significant Hspa1b and Notch3 downregulation and Camkk2 level elevation. Compared with MOD group, INS group showed further downregulation of the Hspa1b level, whereas MID group exhibited an increase. The Camkk2 levels in INS, MID, and HIGH groups were further reduced. The Notch3 levels did not significantly change in INS and MID groups, whereas that of HIGH group increased. Additionally, compared with NC group, MOD group demonstrated upregulation of the Myl1, Mylpf, Acacb, and Pygm levels and downregulation of Fasn level. Compared with MOD group, Myl1, Mylpf, and Pygm levels in INS, MID, and HIGH groups were down-regulated, whereas Fasn and Acacb levels were up-regulated.
BJRD may influence pancreatic β-cell function, thereby enhancing the function of the skeletal muscle and adipose tissues in a T1DM rat model.
白虎加人参汤（BJRD）在临床上用于糖尿病（DM）的治疗。
阐明白虎加人参汤治疗1型糖尿病（T1DM）的潜在机制。
通过腹腔注射链脲佐菌素（STZ）建立T1DM模型。随后将大鼠随机分为正常对照组（NC）、模型组（MOD）、胰岛素组（INS）、胰岛素+白虎加人参汤中剂量组（MID）和胰岛素+白虎加人参汤高剂量组（HIGH）。测量大鼠体重。进行转录组测序以检测肌肉和脂肪组织中差异表达基因（DEGs）。利用定量实时聚合酶链反应验证DEG水平。
与NC组相比，MOD、INS、MID和HIGH组的体重显著降低。与NC组相比，MOD组显示Hspa1b和Notch3显著下调，Camkk2水平升高。与MOD组相比，INS组的Hspa1b水平进一步下调，而MID组则升高。INS、MID和HIGH组的Camkk2水平进一步降低。INS和MID组的Notch3水平无显著变化，而HIGH组升高。此外，与NC组相比，MOD组的Myl1、Mylpf、Acacb和Pygm水平上调，Fasn水平下调。与MOD组相比，INS、MID和HIGH组的Myl1、Mylpf和Pygm水平下调，而Fasn和Acacb水平上调。
白虎加人参汤可能影响胰腺β细胞功能，从而增强T1DM大鼠模型中骨骼肌和脂肪组织的功能。
