Słomnicka Renata, Cieplak Magdalena, Antosiewicz Magda, Sadłos Alicja, Galczak Aleksandra, Kaźmińska Karolina, Bartoszewski Grzegorz
Department of Plant Genetics Breeding and Biotechnology, Institute of Biology, Warsaw University of Life Sciences, SGGW, Warsaw, Poland.
J Appl Genet. 2024 Dec 23. doi: 10.1007/s13353-024-00927-3.
Plant regeneration in tissue cultures is crucial for the application of biotechnological methods to plant breeding. However, the genetic basis of in vitro plant regeneration is not fully understood. For cucumber, regeneration protocols from different types of explants have been reported, but thus far, the molecular basis of regeneration from cotyledon explants has only been studied. The aim of this work was to identify quantitative trait loci (QTLs) for in vitro plant regeneration from cucumber leaf microexplants. Plant regeneration was evaluated using a population of recombinant inbred lines (RILs) developed from a cross between line B10, characterized by high regeneration efficiency, and the low regeneration efficiency line Gy14. All RILs were scored for frequency of callus formation, organogenesis, and shoot regeneration. RILs with regeneration efficiencies higher than that of line B10 have been observed. QTLs for the frequency of organogenesis and shoot regeneration were identified. All the QTLs were mapped on cucumber chromosome 6, explaining 11.9 to 20% of the phenotypic variance. The major-effect QTL for organogenesis or6.1 was located on the upper arm of chromosome 6. The QTLs for shoot regeneration frequency, sr6.1A and sr6.1B, were located on the lower arm of chromosome 6. Analysis of the genomic region corresponding to these QTLs combined with gene expression profiling revealed that CsARF6 and CsWOX9 are gene candidates underlying these QTLs. This study is a step toward identifying the genes controlling the ability of cucumber plant regeneration from leaf explants.
组织培养中的植株再生对于生物技术方法在植物育种中的应用至关重要。然而，体外植株再生的遗传基础尚未完全明确。对于黄瓜，已报道了不同类型外植体的再生方案，但迄今为止，仅对子叶外植体再生的分子基础进行了研究。这项工作的目的是鉴定黄瓜叶片微小外植体体外植株再生的数量性状基因座（QTL）。使用由再生效率高的B10品系与再生效率低的Gy14品系杂交产生的重组自交系（RIL）群体评估植株再生情况。对所有RIL的愈伤组织形成频率、器官发生和芽再生进行评分。已观察到再生效率高于B10品系的RIL。鉴定了器官发生频率和芽再生的QTL。所有QTL均定位在黄瓜6号染色体上，解释了11.9%至20%的表型变异。器官发生的主效QTL or6.1位于6号染色体的长臂上。芽再生频率的QTL，sr6.1A和sr6.1B，位于6号染色体的短臂上。对与这些QTL相对应的基因组区域进行分析并结合基因表达谱分析表明，CsARF6和CsWOX9是这些QTL潜在的候选基因。这项研究朝着鉴定控制黄瓜叶片外植体植株再生能力的基因迈出了一步。
