全游离DNA液体活检对日本口腔鳞状细胞癌患者全身转移的预测效能
The Efficacy of Liquid Biopsy of Total cfDNA for Predicting Systemic Metastasis in Japanese Patients With Oral Squamous Cell Carcinoma.
作者信息
Uchibori Masahiro, Hosomichi Kazuyoshi, Hoshimoto Yasutaka, Sasaki Masashi, Aoki Takayuki, Tajima Atsushi, Ota Yoshihide, Kimura Minoru
机构信息
Department of Oral and Maxillofacial Surgery, Tokai University School of Medicine, Isehara, Japan.
Laboratory of Computational Genomics, School of Life Science, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan.
出版信息
Head Neck. 2025 May;47(5):1411-1420. doi: 10.1002/hed.28054. Epub 2024 Dec 27.
BACKGROUND
The use of liquid biopsy of total cell-free DNA (cfDNA) to identify otherwise undetectable cancers has attracted interest; however, its efficacy remains unknown. We explored whether analysis using total cfDNA is efficacious for Japanese patients with oral squamous cell carcinoma (OSCC).
METHODS
We collected total cfDNA from nine patients with OSCC preoperatively, 1 month postoperatively, and every 3 months thereafter to analyze this association. We used a target DNA sequence for genetic mutation analysis of tumor tissues collected from 33 patients, including the aforementioned nine patients.
RESULTS
Patients with good disease control showed negligible changes in preoperative and postoperative total cfDNA concentrations. A rapid increase in total cfDNA concentration was observed in patients who developed systemic metastases. Patients whose tumor tissue DNA showed genetic mutations had the same mutations in preoperative circulating tumor DNA.
CONCLUSIONS
Our data suggested that analyzing total cfDNA is efficacious for patients with OSCC.
背景
使用全游离细胞DNA（cfDNA）进行液体活检以识别其他情况下无法检测到的癌症已引起关注；然而，其疗效尚不清楚。我们探讨了对日本口腔鳞状细胞癌（OSCC）患者使用全cfDNA进行分析是否有效。
方法
我们收集了9例OSCC患者术前、术后1个月以及此后每3个月的全cfDNA，以分析这种关联。我们使用一个目标DNA序列对从33例患者（包括上述9例患者）收集的肿瘤组织进行基因突变分析。
结果
疾病控制良好的患者术前和术后全cfDNA浓度变化可忽略不计。发生全身转移的患者全cfDNA浓度迅速升高。肿瘤组织DNA显示有基因突变的患者，其术前循环肿瘤DNA中存在相同的突变。
结论
我们的数据表明，对OSCC患者分析全cfDNA是有效的。
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