Belaffif Mohammad B, Brown Morgan C, Marcial Brenda, Baysal Can, Swaminathan Kankshita
HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA; Center for Advanced Bioenergy and Bioproducts Innovation, 1206 W. Gregory Drive (IGB), Urbana, IL 61801, USA; Agria Analytica, CIBIS NINE 11th Floor, Jl.TB Simatupang No.2, Jakarta Selatan, DKI Jakarta 12560, Indonesia.
HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA.
Curr Opin Biotechnol. 2025 Feb;91:103241. doi: 10.1016/j.copbio.2024.103241. Epub 2024 Dec 28.
Plants are an important source of food, energy, and bioproducts. Advances in genetics, genomics-assisted breeding, and biotechnology have facilitated the combining of desirable traits into elite cultivars. To ensure sustainable crop production in the face of climate challenges and population growth, it is essential to develop and implement techniques that increase crop yield and resilience in environments facing water scarcity, nutrient deficiencies, and other abiotic and biotic stressors. Plant transformation and genome editing are critical tools in the development of new cultivars. Here, we discuss recent advances in plant transformation technologies aimed at enhancing efficiency, throughput, and the number of transformable genotypes. These advancements include the use of morphogenic regulators, virus-mediated genetic modifications, and in planta transformation with Rhizobium rhizogenes.
植物是食物、能源和生物产品的重要来源。遗传学、基因组学辅助育种和生物技术的进步促进了将理想性状组合到优良品种中。为了在面对气候挑战和人口增长的情况下确保作物可持续生产，开发和应用能够提高作物产量以及在面临水资源短缺、养分缺乏和其他非生物及生物胁迫的环境中的抗逆性的技术至关重要。植物转化和基因组编辑是培育新品种的关键工具。在此，我们讨论旨在提高效率、通量和可转化基因型数量的植物转化技术的最新进展。这些进展包括使用形态发生调节剂、病毒介导的基因修饰以及用发根农杆菌进行植物体内转化。
