Li Min, Li Wenjie, Wang Chunxue, Ji Lei, Han Kun, Gong Jiahui, Dong Siyuan, Wang Hailong, Zhu Xueming, Du Binghai, Liu Kai, Jiang Juquan, Wang Chengqiang
Shandong Key Laboratory of Agricultural Microbiology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, National Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, China.
Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Front Bioeng Biotechnol. 2024 Dec 13;12:1464195. doi: 10.3389/fbioe.2024.1464195. eCollection 2024.
Saline-alkali land is a type of soil environment that causes poor crop growth and low yields. Its management and utilization are, therefore of great significance for increasing arable land resources, ensuring food security, and enhancing agricultural production capacity. The application of plant growth-promoting rhizobacteria (PGPR) is an effective way to promote the establishment of symbiotic relationships between plants and the rhizosphere microenvironment, plant growth and development, and plant resistance to saline-alkali stress. In this study, multiple saline-alkali-resistant bacteria were screened from a saline-alkali land environment and some of them were found to have significantly promotive effects on the growth of wheat seedlings under saline-alkali stress. Using these PGPR, a compound microbial community was selectively obtained from the root-zone soil environment of wheat seedlings, and the metagenomic sequencing analysis of wheat root-zone soil microbiomes was performed. As a result, a compound microbial agent with a 5-33: S-3: BJYX: G51-1 ratio of 275:63:5:1 was obtained through the self-selection of wheat seedlings. The synthetic compound microbial agent significantly improved the growth of wheat seedlings in saline-alkali soil, as the physiological plant height, aboveground and underground fresh weights, and aboveground and underground dry weights of 21-day-old wheat seedlings were increased by 27.39% ( < 0.01), 147.33% ( < 0.01), 282.98% ( < 0.01), 194.86% ( < 0.01), and 218.60% ( < 0.01), respectively. The promoting effect of this compound microbial agent was also greater than that of each strain on the growth of wheat seedlings. This microbial agent could also regulate some enzyme activities of wheat seedlings and the saline-alkali soil, thereby, promoting the growth of these seedlings. In this study, we analyze an efficient microbial agent and the theoretical basis for promoting the growth of wheat seedlings under saline-alkali stress, thereby, suggesting an important solution for the management and utilization of saline-alkali land.
盐碱地是一种导致作物生长不良和产量低下的土壤环境。因此，其治理与利用对于增加耕地资源、保障粮食安全以及提高农业生产能力具有重要意义。应用植物促生根际细菌（PGPR）是促进植物与根际微环境建立共生关系、促进植物生长发育以及提高植物抗盐碱胁迫能力的有效途径。在本研究中，从盐碱地环境中筛选出多种耐盐碱细菌，发现其中一些对盐碱胁迫下小麦幼苗的生长具有显著促进作用。利用这些PGPR，从小麦幼苗根际土壤环境中选择性地获得了复合微生物群落，并对小麦根际土壤微生物群落进行了宏基因组测序分析。结果，通过小麦幼苗的自我选择，获得了一种比例为275:63:5:1的5-33:S-3:BJYX:G51-1复合微生物制剂。该复合微生物制剂显著促进了盐碱土壤中小麦幼苗的生长，21日龄小麦幼苗的株高、地上和地下鲜重以及地上和地下干重分别增加了27.39%（P<0.01）、147.33%（P<0.01）、282.98%（P<0.01）、194.86%（P<0.01）和218.60%（P<0.01）。这种复合微生物制剂对小麦幼苗生长的促进作用也大于各菌株单独作用的效果。该微生物制剂还可以调节小麦幼苗和盐碱土壤的一些酶活性，从而促进这些幼苗的生长。在本研究中，我们分析了一种高效微生物制剂以及盐碱胁迫下促进小麦幼苗生长的理论依据，从而为盐碱地的治理与利用提出了一个重要的解决方案。
