成年小鼠组织中巨噬细胞总含量的定量分析。使用单克隆抗体F4/80的免疫化学研究。
Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.
作者信息
Lee S H, Starkey P M, Gordon S
出版信息
J Exp Med. 1985 Mar 1;161(3):475-89. doi: 10.1084/jem.161.3.475.
Abstract
We have estimated the macrophage content of different tissues of the normal adult mouse using F4/80, a highly specific antigen marker for mature mouse macrophages. An absorption indirect binding assay was used to quantitate F4/80 antigen against a calibration standard made from the J774.2 macrophage-like cell line. The richest sources of tissue F4/80 antigen were found to be bone marrow, spleen, cervical and mesenteric lymph nodes, large bowel, liver, kidneys, and small bowel. The organs that have the highest total F4/80 antigen content are the liver, large bowel, small bowel, bone marrow, spleen, cervical and mesenteric lymph nodes, and kidney. We conclude that the mononuclear phagocyte system is mainly distributed in the gastrointestinal tract and liver, followed by hemopoietic and lymphoid tissues.
摘要
我们使用F4/80（一种成熟小鼠巨噬细胞的高度特异性抗原标志物）估计了正常成年小鼠不同组织中的巨噬细胞含量。采用吸收间接结合测定法，以J774.2巨噬细胞样细胞系制备的校准标准品对F4/80抗原进行定量。发现组织F4/80抗原最丰富的来源是骨髓、脾脏、颈淋巴结和肠系膜淋巴结、大肠、肝脏、肾脏和小肠。F4/80抗原总含量最高的器官是肝脏、大肠、小肠、骨髓、脾脏、颈淋巴结和肠系膜淋巴结以及肾脏。我们得出结论，单核吞噬细胞系统主要分布在胃肠道和肝脏，其次是造血组织和淋巴组织。
相似文献
1
Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.成年小鼠组织中巨噬细胞总含量的定量分析。使用单克隆抗体F4/80的免疫化学研究。
J Exp Med. 1985 Mar 1;161(3):475-89. doi: 10.1084/jem.161.3.475.
2
F4/80, a monoclonal antibody directed specifically against the mouse macrophage.F4/80，一种特异性针对小鼠巨噬细胞的单克隆抗体。
Eur J Immunol. 1981 Oct;11(10):805-15. doi: 10.1002/eji.1830111013.
3
The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs.通过抗原F4/80免疫组织化学定位确定的小鼠单核吞噬细胞系统。淋巴和造血器官中巨噬细胞、朗格汉斯细胞、网状细胞和树突状细胞之间的关系。
J Exp Med. 1983 Nov 1;158(5):1522-36. doi: 10.1084/jem.158.5.1522.
4
The anatomy of peripheral lymphoid organs with emphasis on accessory cells: light-microscopic immunocytochemical studies of mouse spleen, lymph node, and Peyer's patch.外周淋巴器官的解剖学，重点是辅助细胞：小鼠脾脏、淋巴结和派尔集合淋巴结的光镜免疫细胞化学研究
Am J Anat. 1984 Jul;170(3):465-81. doi: 10.1002/aja.1001700318.
5
Macrophages in haemopoietic and other tissues of the developing mouse detected by the monoclonal antibody F4/80.利用单克隆抗体F4/80检测发育中小鼠造血组织及其他组织中的巨噬细胞。
Development. 1991 Jun;112(2):517-26. doi: 10.1242/dev.112.2.517.
6
Specific antimononuclear phagocyte monoclonal antibodies. Application to the purification of dendritic cells and the tissue localization of macrophages.特异性抗单核吞噬细胞单克隆抗体。在树突状细胞纯化及巨噬细胞组织定位中的应用。
J Exp Med. 1983 Jul 1;158(1):126-45. doi: 10.1084/jem.158.1.126.
7
8
¹¹¹In-anti-F4/80-A3-1 antibody: a novel tracer to image macrophages.¹¹¹In标记抗F4/80-A3-1抗体：一种用于巨噬细胞成像的新型示踪剂。
Eur J Nucl Med Mol Imaging. 2015 Aug;42(9):1430-8. doi: 10.1007/s00259-015-3084-8. Epub 2015 May 27.
9
Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.三色荧光免疫组织化学揭示了小鼠脾脏和肝脏中巨噬细胞标志物染色细胞的多样性。
J Immunol Methods. 2008 May 20;334(1-2):70-81. doi: 10.1016/j.jim.2008.02.005. Epub 2008 Mar 7.
10
Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.小鼠巨噬细胞清道夫受体：在体内的表达及作为淋巴细胞和非淋巴细胞器官中巨噬细胞黏附受体的功能。
Eur J Immunol. 1995 Feb;25(2):466-73. doi: 10.1002/eji.1830250224.
引用本文的文献
1
Citrulline Inhibits Clostridioides difficile Infection With Anti-inflammatory Effects.瓜氨酸通过抗炎作用抑制艰难梭菌感染。
Cell Mol Gastroenterol Hepatol. 2025;19(6):101474. doi: 10.1016/j.jcmgh.2025.101474. Epub 2025 Feb 7.
2
Exploring and Analyzing the Systemic Delivery Barriers for Nanoparticles.探索与分析纳米颗粒的全身递送障碍
Adv Funct Mater. 2024 Feb 19;34(8). doi: 10.1002/adfm.202308446. Epub 2023 Nov 20.
3
Rediscovery of mononuclear phagocyte system blockade for nanoparticle drug delivery.单核吞噬细胞系统阻断在纳米药物递送中的再发现。
Nat Commun. 2024 May 22;15(1):4366. doi: 10.1038/s41467-024-48838-5.
4
The interplay between the microbiota, diet and T regulatory cells in the preservation of the gut barrier in inflammatory bowel disease.微生物群、饮食与调节性T细胞在炎症性肠病肠道屏障保护中的相互作用。
Front Microbiol. 2023 Dec 1;14:1291724. doi: 10.3389/fmicb.2023.1291724. eCollection 2023.
5
Influence of food emulsifiers on cellular function and inflammation, a preliminary study.食品乳化剂对细胞功能和炎症的影响：一项初步研究
Front Nutr. 2023 Aug 2;10:1197686. doi: 10.3389/fnut.2023.1197686. eCollection 2023.
6
Macrophages drive KSHV B cell latency.巨噬细胞驱动卡波西肉瘤相关疱疹病毒 B 细胞潜伏。
Cell Rep. 2023 Jul 25;42(7):112767. doi: 10.1016/j.celrep.2023.112767. Epub 2023 Jul 12.
7
Therapeutic effect of fenofibrate for non-alcoholic steatohepatitis in mouse models is dependent on regime design.非诺贝特对小鼠非酒精性脂肪性肝炎模型的治疗效果取决于给药方案设计。
Front Pharmacol. 2023 May 12;14:1190458. doi: 10.3389/fphar.2023.1190458. eCollection 2023.
8
The Impact of Phase-Specific Macrophage Depletion on Intestinal Anastomotic Healing.特定相巨噬细胞耗竭对肠道吻合口愈合的影响。
Cells. 2023 Mar 29;12(7):1039. doi: 10.3390/cells12071039.
9
The Phylogeny, Ontogeny, and Organ-specific Differentiation of Macrophages in the Developing Intestine.发育中肠道巨噬细胞的系统发育、个体发育及器官特异性分化
Newborn (Clarksville). 2022 Oct-Dec;1(4):340-355. doi: 10.5005/jp-journals-11002-0044. Epub 2022 Dec 23.
10
Mucosal Immunity and the Gut-Microbiota-Brain-Axis in Neuroimmune Disease.黏膜免疫与神经免疫疾病中的肠道微生物群-脑轴
Int J Mol Sci. 2022 Nov 1;23(21):13328. doi: 10.3390/ijms232113328.
本文引用的文献
1
The growth in vitro of single isolated tissue cells.单个分离组织细胞的体外生长。
J Natl Cancer Inst. 1948 Dec;9(3):229-46.
2
3
F4/80, a monoclonal antibody directed specifically against the mouse macrophage.F4/80，一种特异性针对小鼠巨噬细胞的单克隆抗体。
Eur J Immunol. 1981 Oct;11(10):805-15. doi: 10.1002/eji.1830111013.
4
Expression of the macrophage-specific antigen F4/80 during differentiation of mouse bone marrow cells in culture.巨噬细胞特异性抗原F4/80在培养的小鼠骨髓细胞分化过程中的表达。
J Exp Med. 1981 Sep 1;154(3):713-25. doi: 10.1084/jem.154.3.713.
5
Surface properties of bacillus Calmette-Guérin-activated mouse macrophages. Reduced expression of mannose-specific endocytosis, Fc receptors, and antigen F4/80 accompanies induction of Ia.卡介苗激活的小鼠巨噬细胞的表面特性。甘露糖特异性内吞作用、Fc受体和抗原F4/80的表达降低伴随着Ia的诱导。
J Exp Med. 1981 Jul 1;154(1):60-76. doi: 10.1084/jem.154.1.60.
6
A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras.一种利用辐射嵌合体对B和T淋巴细胞干细胞进行再增殖检测的方法。
Cell Tissue Kinet. 1981 Jul;14(4):445-64. doi: 10.1111/j.1365-2184.1981.tb00551.x.
7
Different macrophage populations distinguished by means of fluorescent polysaccharides. Recognition and properties of marginal-zone macrophages.通过荧光多糖区分不同的巨噬细胞群体。边缘区巨噬细胞的识别与特性。
Eur J Immunol. 1981 Mar;11(3):221-8. doi: 10.1002/eji.1830110311.
8
Mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Identification of resident macrophages in renal medullary and cortical interstitium and the juxtaglomerular complex.通过抗原F4/80免疫组织化学定位确定的小鼠单核吞噬细胞系统。肾髓质和皮质间质以及肾小球旁复合体中驻留巨噬细胞的鉴定。
J Exp Med. 1983 May 1;157(5):1704-9. doi: 10.1084/jem.157.5.1704.
9
A quantitative evaluation of pulmonary macrophage kinetics.肺巨噬细胞动力学的定量评估。
Cell Tissue Kinet. 1983 May;16(3):211-9.
10
The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia.通过抗原F4/80的免疫组织化学定位确定的小鼠单核吞噬细胞系统：与上皮相关的巨噬细胞。
Anat Rec. 1984 Nov;210(3):503-12. doi: 10.1002/ar.1092100311.
Zotero 插件