Silva Jéssica Líver Alves, Rezende-Oliveira Karine, da Silva Marcos Vinicius, Gómez-Hernández César, Peghini Bethânea Crema, Silva Neide Maria, Mineo José Roberto, Rodrigues Júnior Virmondes
Course of Tropical Medicine and Infectology, Laboratory of Immunology, Federal University of Triângulo Mineiro, Avenida Frei Paulino, No. 30, 38025-180 Uberaba, MG, Brazil.
Laboratory of Biomedical Sciences, Federal University of Uberlândia, Rua 20, No. 1600, 38304-402 Ituiutaba, MG, Brazil.
Mediators Inflamm. 2014;2014:573825. doi: 10.1155/2014/573825. Epub 2014 Aug 17.
This study aimed to measure the synthesis of Th1 and Th2 cytokines by mononuclear cells after culture with live T. gondii and identified Th17 (CD4(+)) and Tc17 (CD8(+)) cells in toxoplasma-seronegative and toxoplasma-seropositive parturient and nonpregnant women. Cytometric bead arrays were used to measure cytokine levels (IL-2, TNF-α, IFN-γ, IL-4, IL-5, and IL-10); immunophenotyping was used to characterize Th17 and Tc17 cells, and the cells were stained with antibodies against CD4(+) and CD8(+) T cells expressing IL-17. The addition of tachyzoites to cell cultures induced the synthesis of IL-5, IL-10, and TNF-α by cells from seronegative parturient women and of IL-5 and IL-10 by cells from seropositive, nonpregnant women. We observed a lower level of IL-17-expressing CD4(+) and CD8(+) T lymphocytes in cultures of cells from seronegative and seropositive parturient and nonpregnant women that were stimulated with tachyzoites, whereas analysis of the CD4(+) and CD8(+) T cell populations showed a higher level of CD4(+) T cells compared with CD8(+) T cells. These results suggest that the cytokine pattern and IL-17-expressing CD4(+) and CD8(+) T lymphocytes may have important roles in the inflammatory response to T. gondii, thus contributing to the maintenance of pregnancy and control of parasite invasion and replication.
本研究旨在测定单核细胞与活的弓形虫共培养后Th1和Th2细胞因子的合成情况，并鉴定弓形虫血清学阴性和血清学阳性的产妇及非孕妇女中的Th17（CD4(+)）和Tc17（CD8(+)）细胞。采用细胞计数珠阵列法测定细胞因子水平（IL-2、TNF-α、IFN-γ、IL-4、IL-5和IL-10）；采用免疫表型分析来鉴定Th17和Tc17细胞，并用抗表达IL-17的CD4(+)和CD8(+) T细胞的抗体对细胞进行染色。向细胞培养物中添加速殖子可诱导血清学阴性产妇的细胞合成IL-5、IL-10和TNF-α，以及血清学阳性非孕妇女的细胞合成IL-5和IL-10。我们观察到，在用速殖子刺激的血清学阴性和血清学阳性的产妇及非孕妇女的细胞培养物中，表达IL-17的CD4(+)和CD8(+) T淋巴细胞水平较低，而对CD4(+)和CD8(+) T细胞群体的分析显示，CD4(+) T细胞水平高于CD8(+) T细胞。这些结果表明，细胞因子模式以及表达IL-17的CD4(+)和CD8(+) T淋巴细胞可能在对弓形虫的炎症反应中发挥重要作用，从而有助于维持妊娠以及控制寄生虫的侵袭和复制。
