隐匿副拟杆菌新种，从一名特应性皮炎患儿粪便中分离得到。
Parabacteroides absconsus sp. nov., isolated from feces of a child with atopic dermatitis.
作者信息
Chaplin Andrei V, Pikina Alla P, Shcherbakova Victoria A, Sokolova Sofia R, Podoprigora Irina V, Das Milana S, Koshkin Filipp A, Ilyina Galit A, Vasiliadis Iuliia A, Efimov Boris A
机构信息
Pirogov Russian National Research Medical University, Moscow, Russia.
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center "Pushchino Scientific Center for Biological Research, Russian Academy of Sciences", Pushchino, Russia.
出版信息
Arch Microbiol. 2025 Jan 5;207(1):25. doi: 10.1007/s00203-024-04221-y.
An obligate anaerobic, Gram-negative rod-shaped bacterial strain designated AD58 was isolated from the feces of a 3-year-old boy with atopic dermatitis. The closest species is Parabacteroides fecalis with 96.98% 16S rRNA gene identity. The average nucleotide identity value between AD58 and P. fecalis AGMB00274 is 85.0-85.4%. The circular genome sequence is 3.77 Mbp long with 43.5 mol% G + C content. The strain AD58 grows at 32-42 °C, its pH range for growth is 6.0-7.5. No growth is observed in the presence of 1% or higher NaCl concentrations. The major fatty acids are anteiso-C15: 0, iso-C15: 0, and summed feature 11 (iso-C17: 0 3-OH and/or C18: 2 DMA), and the predominant respiratory quinone is MK-9. Conditioned media from AD58 increased expression of IL-8 but decreased expression of TLR-4 in HT29 cells. Based on the described properties, we propose AD58 as the type strain of Parabacteroides absconsus sp. nov. (= VKM B-3630 = JCM 35468).
从一名患有特应性皮炎的3岁男孩粪便中分离出一株专性厌氧、革兰氏阴性杆状细菌菌株，命名为AD58。与其亲缘关系最近的物种是粪副拟杆菌，16S rRNA基因同一性为96.98%。AD58与粪副拟杆菌AGMB00274之间的平均核苷酸同一性值为85.0 - 85.4%。环状基因组序列长3.77 Mbp，G + C含量为43.5 mol%。菌株AD58在32 - 42℃生长，其生长的pH范围为6.0 - 7.5。在NaCl浓度为1%或更高时未观察到生长。主要脂肪酸为anteiso - C15:0、iso - C15:0和特征性总和11（iso - C17:0 3 - OH和/或C18:2 DMA），主要呼吸醌为MK - 9。AD58的条件培养基增加了HT29细胞中IL - 8的表达，但降低了TLR - 4的表达。基于所描述的特性，我们提议将AD58作为隐匿副拟杆菌新种的模式菌株（= VKM B - 3630 = JCM 35468）。
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