Glasser D B, Matsuda M, Ellis J G, Edelhauser H F
Am J Ophthalmol. 1985 Mar 15;99(3):321-8. doi: 10.1016/0002-9394(85)90363-0.
We used wide-field specular microscopy and corneal pachymetry to evaluate the effect of anterior chamber irrigation with BSS and BSS Plus on the corneal endothelium of cats. Endothelial changes were quantitated by computerized morphometric analysis of individual cells. After short-term (15 and 30 minutes) and long-term (one and two hours) irrigation, endothelial cell density remained unchanged. Corneal thickness increased significantly in the BSS group after one hour of irrigation. BSS Plus caused minimal changes in endothelial morphologic characteristics regardless of the irrigation time. By comparison, BSS caused a significant increase in the coefficient of variation of cell area (polymegethism) and a decrease in the percentage of hexagonal cells (pleomorphism). These changes were more prominent after prolonged irrigation. The morphologic changes caused by BSS irrigation are indicative of a stressed endothelial monolayer that may be more susceptible to additional surgical trauma.
我们使用广域镜面显微镜和角膜测厚法来评估用平衡盐溶液（BSS）和复方电解质平衡盐溶液（BSS Plus）进行前房冲洗对猫角膜内皮的影响。通过对单个细胞进行计算机形态计量分析来量化内皮变化。在短期（15分钟和30分钟）和长期（1小时和2小时）冲洗后，内皮细胞密度保持不变。在BSS组冲洗1小时后角膜厚度显著增加。无论冲洗时间如何，BSS Plus引起的内皮形态特征变化最小。相比之下，BSS导致细胞面积变异系数（多形性）显著增加，六边形细胞百分比（异形性）降低。长时间冲洗后这些变化更明显。BSS冲洗引起的形态学变化表明内皮单层受到应激，可能更容易受到额外的手术创伤。
