Aldayel Munirah F
Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
Life (Basel). 2024 Dec 10;14(12):1639. doi: 10.3390/life14121639.
The biosynthesis of silver nanoparticles using plant extracts is a promising field of research because of the useful biomedical applications of metal nanoparticles. In this study, the antibacterial and antioxidant properties of silver nanoparticles biosynthesized with the aqueous leaf extract of were defined using a simple, eco-friendly, consistent, and cost-effective method. The leaf extract of (PT) served as a capping and reducing agent to biosynthesize silver nanoparticles. The effects of several parameters, such as the concentration of AgNO, ratio of AgNO to extract, pH, and incubation time, were examined to optimize the synthesis process. In total, 5 mM of AgNO, a 1:0.06 ratio of AgNO to extract, pH 9.0, and reaction mixture incubation for 24 h were found to be the ideal parameters for biosynthesizing silver nanoparticles (AgNPs). UV-visible spectroscopy, X-ray diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to characterize the biosynthesized silver nanoparticles (PT-AgNPs). Gram-positive bacteria ( and ) and Gram-negative bacteria ( and ) were used to test the PT-AgNPs' antibacterial activity. The presence of different functional groups was determined using FTIR. The AgNPs were hexagon shaped. The nanoparticles were more toxic against than both and . In antioxidant analyses, the AgNPs were found to be as strong at free radical scavenging as gallic acid (standard), with IC values of 0.69 and 22.30 μg/mL for DPPH and ABTS radicals, respectively. Interestingly, the PT-AgNPs displayed increased anti-inflammatory activity compared with the leaf extract (79% vs. 59% at 500 µg/mL). The PT-AgNPs did not display any cytotoxicity against the MCF-7 cell line at the MIC. In conclusion, silver nanoparticles fortified with extract exhibited potential as effective antibacterial, anti-inflammatory, and antioxidant agents, suggesting their viability as alternatives to commercially available products.
由于金属纳米颗粒在生物医学方面的有益应用，利用植物提取物生物合成银纳米颗粒是一个很有前景的研究领域。在本研究中，采用一种简单、环保、稳定且经济高效的方法，确定了用[植物名称]叶水提取物生物合成的银纳米颗粒的抗菌和抗氧化性能。[植物名称]（PT）的叶提取物作为封端剂和还原剂来生物合成银纳米颗粒。研究了几个参数的影响，如硝酸银浓度、硝酸银与提取物的比例、pH值和孵育时间，以优化合成过程。结果发现，5 mM硝酸银、硝酸银与提取物1:0.06的比例、pH 9.0以及反应混合物孵育24小时是生物合成银纳米颗粒（AgNPs）的理想参数。利用紫外可见光谱、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜对生物合成的银纳米颗粒（PT-AgNPs）进行了表征。使用革兰氏阳性菌（[细菌名称1]和[细菌名称2]）和革兰氏阴性菌（[细菌名称3]和[细菌名称4]）测试了PT-AgNPs的抗菌活性。利用FTIR确定了不同官能团的存在。AgNPs呈六边形。纳米颗粒对[细菌名称5]的毒性比对[细菌名称6]和[细菌名称7]更强。在抗氧化分析中，发现AgNPs清除自由基的能力与没食子酸（标准品）一样强，对DPPH和ABTS自由基的IC50值分别为0.69和22.30 μg/mL。有趣的是，与[植物名称]叶提取物相比，PT-AgNPs表现出增强的抗炎活性（500 µg/mL时为79%对59%）。在最低抑菌浓度下，PT-AgNPs对MCF-7细胞系未表现出任何细胞毒性。总之，用[植物名称]提取物强化的银纳米颗粒展现出作为有效抗菌、抗炎和抗氧化剂的潜力，表明它们可作为市售产品的替代品。
