Department of Biotechnology, Faculty of Life Sciences, University of Central Punjab, Lahore 54590, Pakistan.
Pak-Austria Fachhochschule, Institute of Applied Sciences and Technology, Mang, Haripur 22621, Pakistan.
Molecules. 2022 Jun 30;27(13):4226. doi: 10.3390/molecules27134226.
In this study, the antibacterial and antifungal properties of silver nanoparticles synthesized with the aqueous plant extract of leaves were defined using a simplistic, environmentally friendly, reliable, and cost-effective method. The aqueous plant extract of , which served as a capping and reducing agent, was used to biosynthesize silver nanoparticles. UV visible spectroscopy, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and scanning electron microscopy were used to analyze the biosynthesized silver nanoparticles (AgNPs). Gram-positive bacteria ( and ) and Gram-negative bacteria () were used to test the AgNPs' antibacterial activity. The presence of different functional groups was determined by FTIR. The AgNPs were rod-like in shape. The nanoparticles were more toxic against than both and . The AgNPs had IC values of 6.22 and 9.43 and mg/mL on HeLa and MCF-7, respectively, proving their comparatively strong potency against MCF-7. This confirmed that silver nanoparticles had strong antibacterial activity and antiproliferative ability against MCF-7 and HeLa cell lines. The mathematical modeling revealed that the pure nanoparticle had a high heat-absorbing capacity compared to the mixed nanoparticle. This research demonstrated that the biosynthesized AgNPs could be used as an antioxidant, antibacterial, and anticancer agent in the future.
本研究采用简单、环保、可靠且经济高效的方法，确定了由 叶的水提植物提取物合成的银纳米粒子的抗菌和抗真菌特性。 水提植物提取物作为稳定剂和还原剂，用于生物合成银纳米粒子。使用紫外可见分光光度法、X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜分析了生物合成的 银纳米粒子（AgNPs）。革兰氏阳性菌（ 和 ）和革兰氏阴性菌（ ）用于测试 AgNPs 的抗菌活性。FTIR 确定了存在不同的官能团。AgNPs 呈棒状。与 和 相比，这些纳米粒子对 的毒性更大。AgNPs 对 HeLa 和 MCF-7 的 IC 值分别为 6.22 和 9.43 mg/mL，表明其对 MCF-7 的相对较强的效力。这证实了银纳米粒子对 MCF-7 和 HeLa 细胞系具有很强的抗菌活性和抗增殖能力。数学模型表明，与混合纳米粒子相比，纯纳米粒子具有更高的吸热能力。这项研究表明，生物合成的 AgNPs 将来可作为抗氧化剂、抗菌剂和抗癌剂使用。
