Asghar Sumeera, Yu Zhenyang, Zhu Zheng, Zheng Dengyue, Zhao Zimo, Xu Yuming, Liu Xiao, Yuan Chao, Li Yan, Wang Wei, Xu Jianfeng, Teng Huailong, Li Jun, Yang Wen-Chao, Chen Chunli
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
The Key Laboratory of Plant Resources Conservation Germplasm Innovation in Mountainous Region, College of Life Sciences, Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China.
Research (Wash D C). 2025 Jan 7;8:0570. doi: 10.34133/research.0570. eCollection 2025.
The presence of Hg causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg in plant tissues is crucial for the management of Hg pollution; however, the related research is still at its early stage. Herein, a small-molecule amphiphilic fluorescent probe (termed ) was developed for the specific detection of Hg with a high sensitivity (~16 nM). Fluorescent imaging applications with not only detected the dynamic distribution of Hg within plant cells at the subcellular level but also enabled the understanding of cell membrane health under Hg stress. This study introduces a valuable imaging tool for elucidating the molecular mechanism of Hg stress in plants, demonstrating the potential of the application of small-molecule fluorescent probes in plant science.
汞的存在会给植物带来巨大压力，通过扰乱细胞周期分裂、光合作用和离子稳态对植物生长和健康产生不利影响。准确可视化汞在植物组织中的时空分布对于汞污染的治理至关重要；然而，相关研究仍处于早期阶段。在此，开发了一种小分子两亲性荧光探针（称为 ）用于高灵敏度（约16 nM）的汞特异性检测。使用 进行的荧光成像应用不仅在亚细胞水平上检测了植物细胞内汞的动态分布，还能够了解汞胁迫下细胞膜的健康状况。本研究引入了一种有价值的成像工具来阐明植物中汞胁迫的分子机制，展示了小分子荧光探针在植物科学中的应用潜力。
