Kanof P D, Greenwald B S, Mohs R C, Davis K L
Biol Psychiatry. 1985 Apr;20(4):375-83. doi: 10.1016/0006-3223(85)90040-x.
The kinetic parameters of choline uptake into red blood cells from patients with Alzheimer's disease and normal elderly controls were compared. The Kd and Vmax values for choline uptake into red cells were determined based on a kinetic analysis of choline uptake at six different concentrations of labeled extracellular choline. The theoretical choline uptake, representing the initial rate of choline influx into choline-depleted red cells given the plasma choline concentration and the kinetic parameters of choline uptake, was also calculated. Alzheimer's disease patients and normal controls did not differ in any kinetic parameter of choline uptake. Kd and Vmax values for red cell choline uptake were strongly correlated among normal controls, but not among patients with Alzheimer's disease. In addition, among the patients with Alzheimer's disease, the theoretical choline uptake was strongly correlated with the severity of dementia. The possible significance of these findings in relation to altered choline metabolism in Alzheimer's disease is discussed.
比较了阿尔茨海默病患者和正常老年对照者红细胞摄取胆碱的动力学参数。基于对六种不同浓度标记细胞外胆碱摄取胆碱的动力学分析，确定了红细胞摄取胆碱的Kd和Vmax值。还计算了理论胆碱摄取量，它代表了在给定血浆胆碱浓度和胆碱摄取动力学参数的情况下，胆碱流入胆碱耗尽红细胞的初始速率。阿尔茨海默病患者和正常对照者在胆碱摄取的任何动力学参数上均无差异。正常对照者中红细胞胆碱摄取的Kd和Vmax值高度相关，但在阿尔茨海默病患者中并非如此。此外，在阿尔茨海默病患者中，理论胆碱摄取量与痴呆严重程度高度相关。讨论了这些发现与阿尔茨海默病中胆碱代谢改变相关的可能意义。
