Takahashi T, Hirsh A
Biophys J. 1985 Mar;47(3):373-80. doi: 10.1016/S0006-3495(85)83928-X.
Intra- and extracellular phase transitions in human peripheral blood monocyte suspensions with and without the cryoprotectant 1 M dimethylsulfoxide were measured using differential scanning calorimetry. Using an fluorescence diacetate/ethidium bromide assay for membrane integrity and a phagocytosis assay for cell function, it was found that mortality was correlated with several phase transitions under a variety of cooling and warming regimens. As a result of these studies we concluded that: intracellular freezing is lethal, but avoidance of freezing during fast cooling is not sufficient to provide complete protection; a subtle freezing injury in the cryoprotected monocytes can be correlated with a measurable increase in devitrification on warming; and the cell contents form more stable glasses than the Hanks' balanced salt solution with fetal calf serum used as the extracellular medium.
使用差示扫描量热法测量了添加和未添加1 M二甲基亚砜冷冻保护剂的人外周血单核细胞悬液的细胞内和细胞外相变。通过使用荧光素二乙酸酯/溴化乙锭检测膜完整性以及吞噬作用检测细胞功能，发现在各种冷却和升温方案下，死亡率与多个相变相关。基于这些研究结果，我们得出以下结论：细胞内结冰是致命的，但在快速冷却过程中避免结冰不足以提供完全保护；冷冻保护的单核细胞中存在的轻微冷冻损伤可能与升温时可测量的反玻璃化增加有关；并且细胞内容物形成的玻璃态比用作细胞外介质的含有胎牛血清的汉克斯平衡盐溶液更稳定。
