Hawkins Penny, Dooley James, Rodda Jessica, Gilbert Colin
Animals in Science Department, Royal Society for the Prevention of Cruelty to Animals, Horsham, West Sussex, UK.
Department of Pathology, University of Cambridge, Cambridge, UK.
Immunol Cell Biol. 2025 Mar;103(3):293-306. doi: 10.1111/imcb.12847. Epub 2025 Jan 9.
This report presents findings from a group of UK-based researchers with expertise in the use of animal models for bone marrow ablation and reconstitution. The primary aim is to facilitate the implementation of the Three Rs (Replacement, Reduction and Refinement), with an emphasis on refinement. Bone marrow ablation and reconstitution procedures are performed for a number of different purposes and conducted predominantly in mice. These procedures can induce significant suffering, classified as "severe", Category E or Category D/E under European, US and Canadian legislation, respectively. Although severity categorization is not mandated in countries such as Australia and New Zealand, legislation still requires that the level of animal suffering must be minimized to the greatest extent possible. This report identifies specific animal welfare issues and proposes practical measures aimed at reducing both animal use and suffering.
本报告展示了一组英国研究人员的研究结果，这些研究人员在使用动物模型进行骨髓消融和重建方面具有专业知识。主要目的是促进“3R原则”（替代、减少和优化）的实施，重点是优化。骨髓消融和重建程序出于多种不同目的进行，且主要在小鼠身上开展。这些程序会导致严重痛苦，根据欧洲、美国和加拿大的立法，分别被归类为“严重”、E类或D/E类。尽管澳大利亚和新西兰等国家没有强制进行严重程度分类，但立法仍要求必须尽可能最大程度地减少动物的痛苦程度。本报告确定了具体的动物福利问题，并提出了旨在减少动物使用和痛苦的切实可行措施。
