Karatas Ozhan, Ozkaraca Mustafa, Atasoy Mustafa O
Department of Pathology, Sivas Cumhuriyet University Faculty of Veterinary Medicine, Sivas, Türkiye.
Department of Virology, Sivas Cumhuriyet University Faculty of Veterinary Medicine, Sivas, Türkiye.
Vet Med Sci. 2025 Jan;11(1):e70132. doi: 10.1002/vms3.70132.
This study aimed to detect the presence of bovine papillomavirus (BPV) in the testicular tissue of bulls over 1-year old by immunohistochemical, immunofluorescence and molecular assay targeting methods. In addition, γH2AX and cytochrome c expressions were evaluated by immunohistochemical and immunofluorescent methods in samples positive for BPV agent. In this study, 100 testicular specimens that did not show any macroscopic papilloma findings were collected. Testicular tissues were collected and examined using histologically and molecularly methods. In the present study, immunopositivity was detected in 6 (6%) samples with the IHC method and in 11 (11%) samples with the IF method. In addition, BPV positivity was detected in four (4%) samples by the PCR method and four (4%) samples determined to be BPV positive by PCR also showed immunopositivity in IHC and IF methods. γH2AX immunopositivity was seen in two of the BPV-positive samples by the IHC method, and γH2AX immunopositivity was observed in four of the samples determined to be positive for the BPV agent by the IF method. Cytochrome c immunopositivity was found in all the samples determined positive for the BPV agent. When the findings were evaluated, the presence of the BPV agent in the testicular tissues of cattle that did not have macroscopic papillomas was revealed for the first time. It was thought that the BPV agent caused double-stranded DNA breaks and increased cytochrome c expression.
本研究旨在通过免疫组织化学、免疫荧光和分子检测靶向方法，检测1岁以上公牛睾丸组织中牛乳头瘤病毒（BPV）的存在情况。此外，采用免疫组织化学和免疫荧光方法，对BPV检测呈阳性的样本中的γH2AX和细胞色素c表达进行评估。本研究收集了100份未发现任何肉眼可见乳头瘤病变的睾丸标本。采用组织学和分子学方法对睾丸组织进行收集和检测。在本研究中，免疫组织化学方法在6份（6%）样本中检测到免疫阳性，免疫荧光方法在11份（11%）样本中检测到免疫阳性。此外，PCR方法在4份（4%）样本中检测到BPV阳性，经PCR检测确定为BPV阳性的4份样本在免疫组织化学和免疫荧光方法中也显示出免疫阳性。免疫组织化学方法在两份BPV阳性样本中检测到γH2AX免疫阳性，免疫荧光方法在4份经检测确定为BPV阳性的样本中观察到γH2AX免疫阳性。在所有经检测确定为BPV阳性的样本中均发现细胞色素c免疫阳性。在对结果进行评估时，首次发现未出现肉眼可见乳头瘤的牛睾丸组织中存在BPV。据认为，BPV会导致双链DNA断裂并增加细胞色素c的表达。
