Savini Federica, Gallina Laura, Prosperi Alice, Puleio Roberto, Lavazza Antonio, Di Marco Patrizia, Tumino Serena, Moreno Ana, Lelli Davide, Guercio Annalisa, Scagliarini Alessandra
Dipartimento di Scienze Mediche Veterinarie, Università di Bologna, Ozzano Emilia, 40064 Bologna, Italy.
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini", 25124 Brescia, Italy.
Pathogens. 2020 May 30;9(6):429. doi: 10.3390/pathogens9060429.
A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a by Next Generation Sequencing analysis. This was subsequently classified as bovine papillomavirus type 1. Negative staining electron microscopy (EM) analyses produced negative test results for papillomavirus particles. RNA in situ hybridization (ISH) confirmed the presence of BPV-1. The results further confirm the ability of BPVs belonging to the genus to infect distantly related species and to cause lesions that are different from sarcoids.
从一只绵羊的乳房中切除了一个增生性菜花状病变。组织学检查证实该病变在宏观上分类为乳头状瘤，无任何成纤维细胞增殖。聚合酶链反应（PCR）检测显示存在牛乳头瘤病毒（BPV），通过下一代测序分析鉴定进一步证实了这一点。随后将其分类为1型牛乳头瘤病毒。负染色电子显微镜（EM）分析对乳头瘤病毒颗粒的检测结果为阴性。RNA原位杂交（ISH）证实了BPV-1的存在。这些结果进一步证实了属于该属的BPV能够感染远缘物种并引起与肉样瘤不同的病变。
