Rojas Letícia Sanfilippo, de Fernandes Michelle Guzmán, da Motta Andressa Cristina Ramaglia, Carminato Luiza Porteiro, Zanatta Ana Caroline, Lôbo-Hajdu Gisele, Jimenez Paula, Custódio Márcio Reis, Hardoim Cristiane Cassiolato Pires
Graduate Program in Evolution and Diversity, Federal University of ABC, Av. dos Estados, Bairro Bangu, Santo André, São Paulo, 5001, CEP 09210-580, Brazil.
Department of Physiology, Institute of Biosciences, University of São Paulo, Rua do Matão, Travessa 14, 101, São Paulo, CEP 05508-090, Brazil.
Braz J Microbiol. 2025 Mar;56(1):117-136. doi: 10.1007/s42770-024-01588-4. Epub 2025 Jan 10.
Culture-dependent and -independent studies have provided access to symbiont genes and the functions they play for host sponges. Thus, this work investigates the diversity, presence of genes of pharmacological interest, biological activities and metabolome of the bacteria isolated from the sponges Aplysina caissara and Aplysina fulva collected on the southwestern Atlantic Coast. The genes for Polyketide Synthases types I and II and Nonribosomal Peptide Synthetases were screened in more than 200 bacterial strains obtained, from which around 40% were putatively novel. Twenty-two were positive for at least one of the genes screened. Among them, 12 exhibited antimicrobial activities and one inhibited the proliferation of cancer cells. The metabolic profiles of the 22 strains were analyzed by liquid chromatography with tandem mass spectrometry and molecular network. The Global Natural Products Social Molecular Networking MolNetEnhancer workflow provided a more comprehensive understanding of the metabolic profiles. The results revealed the existence of a wide range of metabolites, however more than half of the compounds could not be identified. It was further observed that the metabolic diversity among the strains varied primarily due to the cultivation medium used. Together the results obtained here revealed the pharmacological potential of the bacteria isolated from Aplysina species.
依赖培养和不依赖培养的研究为获取共生体基因及其对宿主海绵所发挥的功能提供了途径。因此，本研究调查了从西南大西洋海岸采集的海绵Caissara海绵（Aplysina caissara）和黄海绵（Aplysina fulva）中分离出的细菌的多样性、具有药理学意义的基因的存在情况、生物活性和代谢组。对所获得的200多个细菌菌株进行了I型和II型聚酮合酶基因以及非核糖体肽合成酶基因的筛选，其中约40%被推测为新基因。22个菌株至少对一种筛选基因呈阳性。其中，12个表现出抗菌活性，1个抑制癌细胞增殖。通过液相色谱-串联质谱和分子网络分析了这22个菌株的代谢谱。全球天然产物社会分子网络MolNetEnhancer工作流程对代谢谱有了更全面的了解。结果显示存在多种代谢物，但超过一半的化合物无法鉴定。进一步观察到，菌株间的代谢多样性主要因所用培养基而异。这里获得的结果共同揭示了从Aplysina属海绵中分离出的细菌的药理学潜力。
