Chen Lu, Liu Lulan, Li Yingyao, Guan Shipeng, Fan Lingling, Qin Xujie, Di Yingtong, Tang Lei, Luo Rongcan, Yan Ying
State Key Laboratory of Functions and Applications of Medicinal Plants & College of Pharmacy, Guizhou Provincial Engineering Technology Research Center for Chemical Drug R&D, Guizhou Medical University, Guiyang 550014, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Int J Mol Sci. 2024 Dec 31;26(1):299. doi: 10.3390/ijms26010299.
Euphjatrophanes H-L (-), four new jatrophane-type and one new lathyrane-type diterpenoid, were isolated from , along with eight known diterpenoids (-). Their structures were established on the basis of extensive spectroscopic analysis and X-ray crystallographic experiments. All compounds were subjected to bioactivity evaluation using flow cytometry in autophagic flux assays with HM mCherry-GFP-LC3 cells, the human microglia cells which stably expressed the tandem monomeric mCherry-GFP-tagged LC3. Compounds -, -, and significantly increase autophagic flux, and compounds and displayed relatively high BBB permeability, with log values of -4.853 and -5.017, respectively. These findings indicated that jatrophane diterpenoids could serve as a valuable source for innovative autophagy inducers.
从[具体来源]中分离出了四种新的麻风树烷型二萜和一种新的山黧豆烷型二萜——喜树欧瑞烷H-L（-），以及八种已知的二萜（-）。它们的结构是通过广泛的光谱分析和X射线晶体学实验确定的。使用稳定表达串联单体mCherry-GFP标记的LC3的人小胶质细胞HM mCherry-GFP-LC3，通过流式细胞术在自噬通量测定中对所有化合物进行生物活性评估。化合物-、-和显著增加自噬通量，化合物和表现出相对较高的血脑屏障通透性，log值分别为-4.853和-5.017。这些发现表明，麻风树烷二萜可作为创新自噬诱导剂的宝贵来源。
