Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science campus 6th floor, Alagappa University, Karaikudi 630004, Tamil Nadu, India; Natural product Biomedical Technology Laboratory, Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon, Gangwon-do 24341, Republic of Korea.
Nanobiosciences and Nanopharmacology Division, Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science campus 6th floor, Alagappa University, Karaikudi 630004, Tamil Nadu, India; Department of Biology, The Gandhigram Rural Institute (Deemed to be University), Gandhigram-624 302, Dindigul District, Tamil Nadu, India.
J Photochem Photobiol B. 2019 Sep;198:111558. doi: 10.1016/j.jphotobiol.2019.111558. Epub 2019 Jul 17.
Facile and low cost garlic clove extract based silver nanoparticles was synthesized and its broad spectrum of therapeutic activity including antibiofilm, antiparasitic and anti-breast cancer activity was evaluated. The synthesized garlic‑silver nanoparticles (G-AgNPs) were characterized by various physico-chemical techniques. G-AgNPs showed good optical property, highly crystalline nature, spherical shape and uniformly dispersed with size measuring between 10 and 50 nm. G-AgNPs have shown greater anti-bacterial and antibiofilm activity on clinically important pathogens methicillin-resistant S. aureus and P. aerigunosa at 100 μg ml. The efficacy of G-AgNPs against earthworm evidenced its effectiveness as anti-helminthic agent in treating intestinal parasites. The significant inhibition of BSA protein denaturation proves its anti-inflammatory property. In addition, G-AgNPs have shown remarkable anticancer effect and significantly inhibited the human breast cancer cell (MCF-7) viability at 100 μg ml after 24 h. A noticeable change in the morphology of MCF-7 cells was also noticed. G-AgNPs were non-toxic to human HEK293 embryonic cells. Also, the non-toxic nature of G-AgNPs to C. cornuta and no morphological, physiological changes proved its safety to the environment. It is concluded that G-AgNPs have a broad range of biological applications and it can be used as an eco-friendly material without having negative effects in the environment.
采用简单且低成本的大蒜鳞茎提取物合成了银纳米粒子，并评估了其广谱的治疗活性，包括抗生物膜、抗寄生虫和抗乳腺癌活性。所合成的大蒜-银纳米粒子（G-AgNPs）通过各种物理化学技术进行了表征。G-AgNPs 表现出良好的光学性质、高结晶性、球形和均匀分散性，粒径在 10 至 50nm 之间。G-AgNPs 在 100μg/ml 时对临床重要病原体耐甲氧西林金黄色葡萄球菌和铜绿假单胞菌表现出更强的抗菌和抗生物膜活性。G-AgNPs 对蚯蚓的功效证明了它作为治疗肠道寄生虫的驱虫剂的有效性。BSA 蛋白变性的显著抑制证明了其抗炎特性。此外，G-AgNPs 还表现出显著的抗癌作用，并在 24 小时后在 100μg/ml 时显著抑制人乳腺癌细胞（MCF-7）的活力。还注意到 MCF-7 细胞形态发生了明显变化。G-AgNPs 对人 HEK293 胚胎细胞没有毒性。此外，G-AgNPs 对 C. cornuta 没有毒性，并且没有形态、生理变化，证明其对环境是安全的。结论是，G-AgNPs 具有广泛的生物学应用，可用作环保材料，而不会对环境产生负面影响。
