Ruenphet Sakchai, Kunanusont Nutnaree, Punyadarsaniya Darsaniya
Department of Animal Biotechnology, Mahanakorn University of Technology, Bangkok, Thailand.
Department of Immunology and Virology, Mahanakorn University of Technology, Bangkok, Thailand.
Vet World. 2024 Nov;17(11):2595-2602. doi: 10.14202/vetworld.2024.2595-2602. Epub 2024 Nov 22.
Potassium peroxymonosulfate (PPMS) is a broad-spectrum disinfectant that oxidizes viral protein capsids. The effectiveness of PPMS in killing viruses depends on several factors, including its concentration, contact time, and present of organic materials. This study evaluated the efficacy of PPMS in an aqueous phase. It also applied PPMS to artificially avian influenza virus (AIV)-contaminated carrier surfaces and clothes and compared its effectiveness with that of sodium dichloroisocyanurate (NaDCC) and quaternary ammonium compounds (QAC).
Four PPMS concentrations (1×, 0.5×, 0.25×, and 0.125×), were evaluated for their virucidal efficacy against Newcastle disease virus (NDV) and AIV in an aqueous phase. The evaluation included testing in the absence and presence of organic materials under different exposure times, such as 5 s, 30 s, 1 min, 3 min, 5 min, 10 min, and 15 min. AIV inactivation was assessed on contaminated carrier surfaces, such as stainless steel, rubber, plastic, and artificially contaminated clothes.
In aqueous phase, concentrations of 1×, 0.5×, 0.25×, and 0.125× inactivated NDV in the absence of organic materials within 5 s, 5 s, 5 min, and 15 min at concentrations of 1×, 0.5×, 0.25×, and 0.125×, respectively. In the presence of organic material contamination, NDV could be inactivated within 30 s for 1×, 1 min for 0.5×, and 10 min for 0.25×; however, 0.125× PPMS did not achieve inactivation within 15 min. PPMS concentrations of 1×, 0.5×, 0.25×, and 0.125× inactivated AIV within 5 s, 5 s, 5 s, and 30 s, respectively, in both the absence and presence of organic materials. PPMS at a concentration of 1× could inactivate AIV on all carriers within 30 s. PPMS at 0.5× and 0.25× concentrations could inactivate AIV within 30 s on rubber and plastic; inactivation occurred within 1 min on stainless steel. However, 0.125× PPMS and 1× QAC could not achieve inactivation within 3 min on all carriers. Finally, PPMS concentrations of 1×, 0.5×, 0.25×, and 0.125× inactivated AIV on rayon sheets within 5 s, 30 s, 5 min, and 15 min, respectively. However, the recommended NaDCC concentration achieved inactivation within 10 min, whereas QAC did not achieve inactivation within 15 min.
PPMS can inactivate enveloped viruses such as NDV and AIV. Furthermore, PPMS is superior to NaDCC and QAC for inactivating viruses on various carrier surfaces and artificially contaminated clothes. However, the virucidal efficacy of PPMS depends on the optimal concentration, organic material conditions, and exposure/contact timing. Therefore, PPMS is a promising alternative disinfectant crucial for enhancing biosecurity and controlling viruses that contaminate animal farms, slaughterhouses, and hospitals.
过一硫酸钾（PPMS）是一种能氧化病毒蛋白衣壳的广谱消毒剂。PPMS杀灭病毒的效果取决于多种因素，包括其浓度、接触时间以及有机物质的存在情况。本研究评估了水相中PPMS的效力。还将PPMS应用于人工感染禽流感病毒（AIV）的载体表面和衣物，并将其效果与二氯异氰尿酸钠（NaDCC）和季铵化合物（QAC）进行比较。
评估了四种PPMS浓度（1倍、0.5倍、0.25倍和0.125倍）对水相中新城疫病毒（NDV）和AIV的杀病毒效力。评估内容包括在有无有机物质存在的情况下，于不同暴露时间（如5秒、30秒、1分钟、3分钟、5分钟、10分钟和15分钟）进行测试。在受污染的载体表面（如不锈钢、橡胶、塑料）和人工污染的衣物上评估AIV的灭活情况。
在水相中，1倍、0.5倍、0.25倍和0.125倍浓度的PPMS在无有机物质存在时，分别在5秒、5秒、5分钟和15分钟内使NDV灭活。在存在有机物质污染的情况下，1倍浓度的PPMS可在30秒内使NDV灭活，0.5倍浓度的在1分钟内，0.25倍浓度的在10分钟内；然而，0.125倍浓度的PPMS在15分钟内未实现灭活。1倍、0.5倍、0.25倍和0.125倍浓度的PPMS在有无有机物质存在的情况下，分别在5秒、5秒、5秒和30秒内使AIV灭活。1倍浓度的PPMS可在30秒内使所有载体上的AIV灭活。0.5倍和0.25倍浓度的PPMS可在30秒内使橡胶和塑料上的AIV灭活；在不锈钢上1分钟内实现灭活。然而，0.125倍浓度的PPMS和1倍浓度的QAC在3分钟内无法使所有载体上的AIV灭活。最后，1倍、0.5倍、0.25倍和0.125倍浓度的PPMS分别在5秒、30秒、5分钟和15分钟内使粘胶纤维布上的AIV灭活。然而，推荐浓度的NaDCC在10分钟内实现灭活，而QAC在15分钟内未实现灭活。
PPMS可灭活包膜病毒，如NDV和AIV。此外，在使各种载体表面和人工污染衣物上的病毒灭活方面，PPMS优于NaDCC和QAC。然而，PPMS的杀病毒效力取决于最佳浓度、有机物质条件以及暴露/接触时间。因此，PPMS是一种有前景的替代消毒剂，对于加强生物安全以及控制污染动物养殖场、屠宰场和医院的病毒至关重要。
