Tian Shiji, Yuan Ruizhong, Ma Xingzhou, Shu XiaoHan, Fang Yu, Lu Zhaohe, Qu Qiuyu, Jin Yu, Gong Jiabao, Peng Yanlin, Wang Ziqi, Ye Xiqian, Chen Xuexin, Tang Pu
State Key Lab of Rice Biology, Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects, and Zhejiang Provincial Key Laboratory of Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, 310058, China.
Institute of insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
Sci Data. 2025 Jan 21;12(1):125. doi: 10.1038/s41597-025-04475-1.
Meteorus pulchricornis Wesmael (Hymenoptera: Braconidae) is an important parasitoid of lepidopteran insects. So far, only three scaffold-level genomes have been published for the genus Meteorus. In this study, we present a high-quality, chromosome-level genome assembly of M. pulchricornis, characterized by high accuracy and contiguity. This assembly was achieved using Oxford Nanopore Technologies long-read, MGI-SEQ short-read, and Hi-C sequencing methods. The final assembly was 158.5 Mb in genome size, with 153.8 Mb (97.03%) assigned to ten pseudochromosomes. The scaffold N50 length reached 17.51 Mb, and the complete Benchmarking Universal Single-Copy Orthologs (BUSCO) score was 99.3%. The genome contains 28.29 Mb of repetitive elements, accounting for 18.39% of the total genome size. We identified 12,342 protein-coding genes, of which 12,308 genes were annotated functionally. Our investigation into gene family evolution in M. pulchricornis showed that 563 gene families expanded, 1,739 contracted, and 58 underwent rapid evolution. The high-quality genome assembly we report here is advantageous for further research on parasitoid wasps and provides a foundational data resource for natural enemy studies.
丽蝇蛹集金小蜂（膜翅目：茧蜂科）是鳞翅目昆虫的一种重要寄生蜂。到目前为止，集金小蜂属仅公布了三个支架水平的基因组。在本研究中，我们展示了丽蝇蛹集金小蜂高质量的染色体水平基因组组装，其特点是准确性和连续性高。该组装使用牛津纳米孔技术长读长、MGI-SEQ短读长和Hi-C测序方法完成。最终组装的基因组大小为158.5 Mb，其中153.8 Mb（97.03%）被分配到10条假染色体上。支架N50长度达到17.51 Mb，完整的基准通用单拷贝直系同源基因（BUSCO）得分是99.3%。基因组包含28.29 Mb的重复元件，占基因组总大小的18.39%。我们鉴定出12342个蛋白质编码基因，其中12308个基因有功能注释。我们对丽蝇蛹集金小蜂基因家族进化的研究表明，有563个基因家族扩张，1739个收缩，58个经历了快速进化。我们在此报告的高质量基因组组装有利于对寄生蜂的进一步研究，并为天敌研究提供了基础数据资源。
