Zhang Zhicheng, Li Haiyang, Zhou Ning, Zheng Zhi, Zhai Tianyou, Xia Fan, Lou Xiaoding
State Key Laboratory of Biogeology and Environmental Geology, Engineering Research Center of Nano-Geomaterials of the Ministry of Education, Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, P. R. China.
State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology (HUST), Wuhan 430074, P. R. China.
Anal Chem. 2025 Feb 4;97(4):1951-1959. doi: 10.1021/acs.analchem.4c04178. Epub 2025 Jan 23.
Proteins have been one of the most important biomarkers for diagnosing diseases, and field-effect transistor (FET) biosensors possess high sensitivity; are label-free; and feature real-time detection, rapidity, and easy integration for protein detection. FET biosensors are mainly made up of FET parts, such as channel materials, and bio parts, such as receptors. This Tutorial provides an in-depth exploration of FET biosensors for protein detection from the composition perspective and discusses the commercialization of point-of-care diagnostics of proteins based on FET biosensors.
蛋白质一直是疾病诊断中最重要的生物标志物之一，而场效应晶体管（FET）生物传感器具有高灵敏度、无需标记，并且具备蛋白质检测的实时检测、快速性和易于集成等特点。FET生物传感器主要由FET部件（如通道材料）和生物部件（如受体）组成。本教程从组成角度深入探讨了用于蛋白质检测的FET生物传感器，并讨论了基于FET生物传感器的蛋白质即时诊断的商业化。
