Barna Bianca, Caldas Lhaís Araújo, Monteiro Jackson, Dos Santos Augusto Leonardo, Pascon Renata Castiglioni, Vallim Marcelo Afonso, Ferreira Marcelo José Pena, Gonçalves Sarah Santos, Dos Santos Glaucia Queiroz, Rodrigues Anderson Messias, de Carvalho Jamile Ambrósio, de Vasconcellos Suzan Pantaroto, Sartorelli Patricia
Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, Diadema 09972-270, SP, Brazil.
Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo 05508-090, SP, Brazil.
J Fungi (Basel). 2024 Dec 31;11(1):22. doi: 10.3390/jof11010022.
Endophytic fungi are increasingly being recognized for their diverse metabolites that may exhibit antimicrobial properties. In our study, we isolated seven endophytic fungal strains from , which were identified as , , , , , and (with two isolates from the same species). Furthermore, the antimicrobial activity of the extracts was assessed, revealing that the extract from demonstrated activity against . Further investigation of secondary metabolites, employing UHPLC-HR-ESI-MS/MS in combination with molecular networking, facilitated annotation of the nine compounds. Of these, five were identified based on matches with the GNPS spectral library, and four were predicted based on the molecular network. Notably, from the extract of , two pairs of diastereoisomeric acyl-tetronic acid derivatives were isolated and characterized using MS and NMR spectroscopy. This study highlights the potential of endophytic fungi as a valuable source of novel antimicrobial agents.
内生真菌因其多样的可能具有抗菌特性的代谢产物而越来越受到认可。在我们的研究中，我们从[具体来源未给出]分离出七株内生真菌菌株，它们被鉴定为[具体真菌名称1]、[具体真菌名称2]、[具体真菌名称3]、[具体真菌名称4]、[具体真菌名称5]和[具体真菌名称6]（有两个分离株来自同一物种）。此外，对提取物的抗菌活性进行了评估，结果表明来自[具体真菌名称]的提取物对[具体受试对象]表现出活性。利用超高效液相色谱 - 高分辨电喷雾电离串联质谱（UHPLC - HR - ESI - MS/MS）结合分子网络对次生代谢产物进行进一步研究，有助于对九种化合物进行注释。其中，五种是基于与全球天然产物社会分子网络光谱库（GNPS）的匹配鉴定出来的，四种是基于分子网络预测的。值得注意的是，从[具体真菌名称]的提取物中，分离出两对非对映异构的酰基 - 四羟基酸衍生物，并通过质谱和核磁共振光谱对其进行了表征。这项研究突出了内生真菌作为新型抗菌剂宝贵来源的潜力。
