Dokuta Sirikwan, Yadoung Sumed, Hongjaisee Sayamon, Khamnoi Phadungkiat, Manochomphu Sirinya, Chuttong Bajaree, Hongsibsong Surat
School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Environmental Sciences Program, Faculty of Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Foods. 2025 Jan 8;14(2):174. doi: 10.3390/foods14020174.
Antibiotic-resistant bacteria are usually found in food-producing animals worldwide. Ciprofloxacin, an antibiotic, can lead to antibiotic residues in food products, posing health risks to consumers and contributing to the development of antimicrobial resistance. Foodborne illnesses occur when adequate attention is not paid to food hygiene and safety, raising the potential for resistant bacteria to spread to humans through the food chain. This study aims to determine the presence of antibiotic-resistant organism contamination and ciprofloxacin residue in raw pork and chicken. Forty-three pork and 33 chicken meat samples were collected from fresh markets in Chiang Mai, Thailand. Antibiotic-resistant organisms were detected by microbial culture and identified by MALDITOF-MS. The antimicrobial sensitivity tests were used to confirm antibiotic resistance. The ciprofloxacin was detected by using an immunochromatographic-based test kit for screening. The results found Extended Spectrum β-lactamase (ESBL)-producing and were detected at 46.51% and 9.30% in pork and 69.70% and 6.06% in chicken meat samples, respectively. Moreover, ciprofloxacin residues were detected in nine samples (11.84%). Based on this study's findings, the people who are involved in the food chain must be concerned about food safety and food hygiene.
耐抗生素细菌在全球范围内的食用动物中普遍存在。环丙沙星是一种抗生素，可导致食品中出现抗生素残留，对消费者健康构成风险，并促使抗菌药物耐药性的发展。如果对食品卫生和安全不够重视，就会发生食源性疾病，增加耐药细菌通过食物链传播给人类的可能性。本研究旨在确定生猪肉和鸡肉中耐抗生素生物体污染及环丙沙星残留的情况。从泰国清迈的新鲜市场采集了43份猪肉和33份鸡肉样本。通过微生物培养检测耐抗生素生物体，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）进行鉴定。采用抗菌药物敏感性试验来确认抗生素耐药性。使用基于免疫层析的检测试剂盒进行环丙沙星筛查。结果发现，产超广谱β-内酰胺酶（ESBL）的情况在猪肉样本中分别为46.51%和9.30%，在鸡肉样本中分别为69.70%和6.06%。此外，在9个样本（11.84%）中检测到环丙沙星残留。基于本研究结果，参与食物链的人员必须关注食品安全和食品卫生。
