Ji Xiang, Chen Shenghua, Wu Qifang, Ling Menglai, Tong Jingyang, Tong Haibin, Wang Guanhua, Gong Jiancheng
Department of Chinese Osteo-traumatology, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou 325200, China.
College of Life and Environmental Science, Wenzhou University, Wenzhou 325000, China.
Int J Biol Macromol. 2025 Apr;300:140235. doi: 10.1016/j.ijbiomac.2025.140235. Epub 2025 Jan 24.
Fatigue is a pathological state that can impair physical and cognitive performance, making the development of effective therapeutic strategies crucial. In this study, an acid polysaccharide (MHa) was isolated from Mentha haplocalyx. Structural analysis showed that MHa (40.7 kDa) has a backbone consisting of 4-α-GalAp, 6-α-Galp, and 4,6-α-Galp, with branches at the C6 of 4,6-α-Galp linked to four distinct side chains, including 4-α-Galp, 3,6-β-Manp, t-α-Araf, t-α-Rhap, t-α-Glcp, and t-β-Rhap. MHa possesses a triple-helix conformation with a sheet-like appearance, which may contribute to its biological stability and activity. Functionally, MHa exhibited significant antifatigue effects, with the 400 mg/kg dose showing the most potent activity. Compared to the model group, treatment with 400 mg/kg of MHa increased the exhaustive swimming time by 1.89-fold in fatigued mice, reduced blood lactate and urea nitrogen levels by 24.21 % and 35.57 %, respectively, and enhanced liver glycogen, muscle glycogen, and ATP levels by 20.08 %, 46.52 %, and 50.43 %, respectively. MHa improved the activities of Ca-Mg-ATPase and Na-K-ATPase, while also enhancing antioxidant defense. Mechanistically, MHa promotes mitochondrial biogenesis and enhances oxidative defense via activating AMPK. These findings highlight the potential of MHa as a promising candidate for developing antifatigue supplements, offering a novel strategy to mitigate fatigue.
疲劳是一种病理状态，会损害身体和认知能力，因此制定有效的治疗策略至关重要。在本研究中，从薄荷中分离出一种酸性多糖（MHa）。结构分析表明，MHa（40.7 kDa）具有由4-α-半乳糖醛酸、6-α-半乳糖和4,6-α-半乳糖组成的主链，在4,6-α-半乳糖的C6处有分支，连接到四个不同的侧链，包括4-α-半乳糖、3,6-β-甘露糖、t-α-阿拉伯糖、t-α-鼠李糖、t-α-葡萄糖和t-β-鼠李糖。MHa具有呈片状外观的三螺旋构象，这可能有助于其生物学稳定性和活性。在功能上，MHa表现出显著的抗疲劳作用，400 mg/kg剂量显示出最有效的活性。与模型组相比，用400 mg/kg的MHa处理使疲劳小鼠的力竭游泳时间增加了1.89倍，分别使血乳酸和尿素氮水平降低了24.21%和35.57%，并分别使肝糖原、肌糖原和ATP水平提高了20.08%、46.52%和50.43%。MHa提高了Ca-Mg-ATP酶和Na-K-ATP酶的活性，同时也增强了抗氧化防御能力。从机制上讲，MHa通过激活AMPK促进线粒体生物发生并增强氧化防御。这些发现突出了MHa作为开发抗疲劳补充剂的有前途候选物的潜力，为减轻疲劳提供了一种新策略。
