代谢组学导向发现双极内酯H - O，来自海洋来源真菌新种的具有抗菌活性的新型蛇孢菌素型倍半萜烯
Metabolomics-Guided Discovery of Bipolarolides H-O, New Ophiobolin-Type Sesterterpenes with Antibacterial Activity from the Marine-Derived Fungus sp. nov.
作者信息
Maharjan Sailesh, Isaksson Johan Mattias, Krupova Monika, Rämä Teppo, Hansen Kine Østnes, Hammer Andersen Jeanette, Hansen Espen Holst
机构信息
Marbio, Norwegian College of Fishery Science (NFH), Faculty of Biosciences, Fisheries, and Economics, UiT-The Arctic University of Norway, Tromsø 9037, Norway.
Department of Pharmacy (IFA), Faculty of Health Sciences, UiT-The Arctic University of Norway, Tromsø 9037, Norway.
出版信息
J Nat Prod. 2025 Feb 28;88(2):349-360. doi: 10.1021/acs.jnatprod.4c01105. Epub 2025 Jan 30.
A marine-derived Pleosporales fungus, , was isolated from driftwood and described as a new species. The fungus was cultivated in liquid media and a molecular networking-driven approach was used to identify potential new secondary metabolites. The targeted compounds were isolated using preparative HPLC-MS, and through extensive spectroscopic analysis, eight new ophiobolin-type sesterterpenes, bipolarolides H-O (-), were identified. The absolute configurations of the compounds were determined by ECD assessment. Bipolarolide L (), M (), and O () exhibited inhibitory activity against with MIC values of 86, 66, and 64 μM, respectively.
从浮木中分离出一种源自海洋的格孢腔菌目真菌，并将其描述为一个新物种。该真菌在液体培养基中培养，并采用分子网络驱动的方法来鉴定潜在的新次级代谢产物。使用制备型高效液相色谱-质谱联用仪分离目标化合物，并通过广泛的光谱分析，鉴定出8种新的蛇孢菌素型倍半萜烯类化合物，即双极内酯H - O（-）。通过电子圆二色光谱（ECD）评估确定了这些化合物的绝对构型。双极内酯L（）、M（）和O（）对[具体对象未给出]表现出抑制活性，其最小抑菌浓度（MIC）值分别为86、66和64 μM。
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