Shim Minki, Jeong Yooseong, Lee Dong-Kyu
College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
Methods Mol Biol. 2025;2895:99-109. doi: 10.1007/978-1-0716-4350-1_8.
This chapter presents a comprehensive approach to profiling plant-derived primary metabolites using metabolomics, highlighting its critical role in decoding the biosynthesis of bioactive plant compounds. It details the utilization of gas chromatography-mass spectrometry (GC-MS) for the effective analysis and profiling of these metabolites. The process, encompassing extraction methods, chemical derivatization, and data processing, is thoroughly outlined. This methodology outlines comprehensive procedures for each stage of the workflow, encompassing metabolite extraction, GC-MS analysis, and data alignment, to produce a metabolomics dataset.
本章介绍了一种使用代谢组学对植物源初级代谢产物进行分析的综合方法，突出了其在解读生物活性植物化合物生物合成过程中的关键作用。详细阐述了利用气相色谱 - 质谱联用仪（GC-MS）对这些代谢产物进行有效分析和分析的方法。全面概述了包括提取方法、化学衍生化和数据处理在内的过程。该方法概述了工作流程每个阶段的综合程序，包括代谢物提取、GC-MS分析和数据比对，以生成代谢组学数据集。
