Syaify Ahmad, Sari Rezmelia, Alhasyimi Ananto A
Department of Periodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Department of Orthodontics, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.
J Taibah Univ Med Sci. 2024 Jul 8;19(4):746-752. doi: 10.1016/j.jtumed.2024.06.007. eCollection 2024 Aug.
This research was aimed at investigating the effects of 70% ethanolic flower extract on cyclooxygenase-2 (COX-2) expression in the gingival epithelium in rats with diabetic periodontitis.
Diabetes and periodontitis were induced in 32 male individuals weighing 200-300 g each. Streptozotocin dissolved in 1 mL citrate buffer was injected intraperitoneally to elicit hyperglycemia. Three days after diabetes induction, the rats' fasting blood glucose levels were measured with a GCU EasyTouch® glucometer. Diabetes was confirmed by fasting blood glucose levels exceeding 200 mg/dL. After diagnosis of diabetic periodontitis, a daily injection of 70% ethanolic extract (n = 16) and saline (n = 16) was intraperitoneally administered for 7 days. Immunohistochemistry was used to detect COX-2 expression in the gingival epithelium on days 1, 3, 5, and 7 after injection, and the number of positively colored cells was expressed as a percentage. Brownish cytoplasm in the gingival epithelium was considered to indicate positive COX-2 expression, which extended from the basal layer to the corneum. The percentage of immunopositive cells was analyzed with two-way ANOVA followed by post-hoc LSD analysis at a 95% significance level.
Injection of 70% ethanolic extract of flower, compared with saline, resulted in greater COX-2 expression on days 1-5. On day 7, however, the group exhibited substantially lower COX-2 expression than the saline group ( < 0.05).
In diabetic periodontitis, 70% ethanolic extract was found to be a useful active component for host modulation therapy. The 70% ethanolic extract of flower modulated COX-2 expression in the gingival epithelium in rats with diabetic periodontitis.
本研究旨在探讨70%乙醇花提取物对糖尿病性牙周炎大鼠牙龈上皮中环氧合酶-2（COX-2）表达的影响。
选取32只体重200 - 300 g的雄性个体诱导糖尿病和牙周炎。将链脲佐菌素溶解于1 mL柠檬酸盐缓冲液中腹腔注射以引发高血糖。糖尿病诱导3天后，用GCU EasyTouch®血糖仪测量大鼠空腹血糖水平。空腹血糖水平超过200 mg/dL则确诊为糖尿病。诊断为糖尿病性牙周炎后，腹腔注射70%乙醇提取物（n = 16）和生理盐水（n = 16），每日1次，共7天。在注射后第1、3、5和7天，采用免疫组织化学法检测牙龈上皮中COX-2的表达，阳性染色细胞数用百分比表示。牙龈上皮中呈褐色的细胞质被认为表明COX-2表达阳性，其从基底层延伸至角质层。采用双向方差分析，然后在95%显著性水平下进行事后LSD分析，分析免疫阳性细胞的百分比。
与生理盐水相比，注射70%乙醇花提取物在第1 - 5天导致更高的COX-2表达。然而，在第7天，该组的COX-2表达明显低于生理盐水组（P < 0.05）。
在糖尿病性牙周炎中，发现70%乙醇提取物是宿主调节治疗的有用活性成分。70%乙醇花提取物调节糖尿病性牙周炎大鼠牙龈上皮中COX-2的表达。
