Smith P F, Langworthy T A, Mayberry W R
J Bacteriol. 1976 Mar;125(3):916-22. doi: 10.1128/jb.125.3.916-922.1976.
Polymeric carbohydrates containing glycerol and fatty acids were isolated from whole cells and membranes of mycoplasmas by hot aqueous phenol extraction and gel filtration. Lipopolysaccharides were found to occur in four species of Acholeplasma, two of Anaeroplasma, and in Mycoplasma neurolyticum. None were detected in Spiroplasma citri or in five species of Mycoplasma. All lipopolysaccharides contained both neutral and N-acylated amino sugars in ratios varying from 1:1 to 3:1. The neutral sugars found in varying distribution were glucose, galactose, and mannose. The amino sugars included fucosamine, an unidentified deoxyhexosamine, galactosamine, and glucosamine. Fucosamine and glucose were the only sugars common to all lipopolysaccharides. The fatty acids were similar to those found in the lipids of each organism.
通过热酚水萃取和凝胶过滤从支原体的全细胞和细胞膜中分离出含有甘油和脂肪酸的聚合碳水化合物。发现脂多糖存在于四种无胆甾原体、两种厌氧支原体以及溶神经支原体中。在柑橘螺原体或五种支原体中未检测到脂多糖。所有脂多糖都含有中性糖和N - 酰化氨基糖,其比例在1:1至3:1之间变化。分布各异的中性糖有葡萄糖、半乳糖和甘露糖。氨基糖包括岩藻糖胺、一种未鉴定的脱氧己糖胺、半乳糖胺和葡萄糖胺。岩藻糖胺和葡萄糖是所有脂多糖共有的唯一糖类。脂肪酸与在每种生物体的脂质中发现的脂肪酸相似。