眼支原体的脂质和脂多糖组成

Lipid and lipopolysaccharide composition of Acholeplasma oculi.

作者信息

Al-Shammari A J, Smith P F

出版信息

J Bacteriol. 1979 Aug;139(2):356-61. doi: 10.1128/jb.139.2.356-361.1979.

DOI:10.1128/jb.139.2.356-361.1979

PMID:457607

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216876/

Abstract

The total lipid content of Acholeplasma oculi comprises 13.3% of the dry weight of the organism and is about equally distributed between the neutral lipids plus glycolipids and the phospholipids. The phospholipids were identified as phosphatidyl glycerol and diphosphatidyl glycerol. The glycolipid fraction contained O-alpha-D-glucopyranosyl-(1 leads to 1)-2,3-diacyl glycerol and O-alpha-D-glucopyranosyl-(1 leads to 2)-O-alpha-D-glucopyranosyl-(1 leads to 1)-2,3-diacyl glycerol. The neutral lipid contained pigmented carotenoids. Hot aqueous phenol extraction of lipid-extracted whole cells yielded a polymeric carbohydrate comprising 2.3% of the dry weight of the organism. The A. oculi lipopolysaccharide was found to contain only neutral sugars and no amino sugar, in contrast to other acholeplasmas. The neutral sugars consisted of fucose, galactose, and glucose in a ratio of 2:19:3.

摘要

眼无胆甾原体的总脂质含量占该生物体干重的13.3%，并且在中性脂质加糖脂和磷脂之间大致平均分布。磷脂被鉴定为磷脂酰甘油和二磷脂酰甘油。糖脂部分包含O-α-D-吡喃葡萄糖基-(1→1)-2,3-二酰基甘油和O-α-D-吡喃葡萄糖基-(1→2)-O-α-D-吡喃葡萄糖基-(1→1)-2,3-二酰基甘油。中性脂质含有色素类胡萝卜素。用热的苯酚水溶液提取脂质提取后的全细胞，得到一种占生物体干重2.3%的聚合碳水化合物。与其他无胆甾原体相比，发现眼无胆甾原体的脂多糖仅含有中性糖而不含氨基糖。中性糖由岩藻糖、半乳糖和葡萄糖以2:19:3的比例组成。