Mehmethanoglu Iklima, Ozakar Emrah, Ozakar Rukiye Sevinc, Ozakar Nurcan, Ruzgar Damla, Gormez Arzu
Research Assistant, Department of Restorative Dentistry, Faculty of Dentistry, Atatürk University, Erzurum, TR- 25240, Turkey.
Associate Professor, Department of Pharmaceutical Technology, Faculty of Pharmacy, Atatürk University, Erzurum, TR-25240, Turkey.
Clin Oral Investig. 2025 Jan 31;29(2):100. doi: 10.1007/s00784-025-06175-x.
This study investigated the use of oral rinse solutions formulated with boron-containing compounds, known for their antibacterial activity, as an alternative to chlorhexidine (CHX).
Boron nitride (BN), boric acid (BA) and sodium borate (SB) were used in the study. BN was used in nanosuspension (BN-NS) due to its low solubility in water. BA and SB were also prepared and used in solution form (BA-S, SB-S). Antibacterial activity against cariogenic bacteria was evaluated using agar well diffusion and microdilution techniques, and antibiofilm activity was evaluated using the crystal violet method. Cytotoxicity in human gingival cell lines (HGF-1) was evaluated using MTT and LDH assays. Surface hardness was measured with Vicker's Microhardness tester and surface roughness was measured with a profilometer. CHX was used as the control group.
BN-NS, BA-S, and SB-S showed antibacterial and antibiofilm activity against all cariogenic bacteria. BN-NS and SB-S demonstrated much lower cytotoxicity compared to CHX, while BA-S exhibited similar cytotoxicity. SB-S caused an increase in enamel surface hardness (p = 0.017), whereas no significant changes were observed in surface roughness or hardness in the other groups (p > 0.05).
BN-NS and SB-S showed antibacterial effects and antibiofilm activity on all tested cariogenic bacteria. BN-NS and SB-S showed lower cytotoxicity than CHX, and their effects on enamel and composite resin surfaces were similar to the control group. Further studies are needed to support the use of BN-NS and SB-S as alternatives to CHX.
BN-NS and SB-S are promising alternatives to the antibacterial agent CHX, which is known to be cytotoxic.
本研究调查了含硼化合物配制的口腔冲洗液作为洗必泰（CHX）替代品的用途，含硼化合物以其抗菌活性而闻名。
本研究使用了氮化硼（BN）、硼酸（BA）和硼酸钠（SB）。由于BN在水中溶解度低，因此以纳米悬浮液（BN-NS）形式使用。BA和SB也被制备并以溶液形式（BA-S、SB-S）使用。使用琼脂孔扩散法和微量稀释技术评估对致龋菌的抗菌活性，并使用结晶紫法评估抗生物膜活性。使用MTT和LDH测定法评估人牙龈细胞系（HGF-1）中的细胞毒性。用维氏显微硬度计测量表面硬度，用轮廓仪测量表面粗糙度。CHX用作对照组。
BN-NS、BA-S和SB-S对所有致龋菌均表现出抗菌和抗生物膜活性。与CHX相比，BN-NS和SB-S的细胞毒性低得多，而BA-S表现出相似的细胞毒性。SB-S使牙釉质表面硬度增加（p = 0.017），而其他组的表面粗糙度或硬度未观察到显著变化（p>0.05）。
BN-NS和SB-S对所有测试的致龋菌均表现出抗菌作用和抗生物膜活性。BN-NS和SB-S的细胞毒性低于CHX，它们对牙釉质和复合树脂表面的影响与对照组相似。需要进一步研究以支持将BN-NS和SB-S用作CHX的替代品。
BN-NS和SB-S是已知具有细胞毒性的抗菌剂CHX的有前途的替代品。
