Huang Tingting, Men Wenjin, Myanganbayar Ariuntungalag, Davaasambuu Undarmaa
Laboratory of Applied Biological Control, School of Agroecology, Mongolian University of Life Sciences, Ulaanbaatar, Mongolia.
Front Plant Sci. 2025 Jan 15;15:1517851. doi: 10.3389/fpls.2024.1517851. eCollection 2024.
L. () is an aromatic plant of the genus, renowned for its medicinal and economic importance. The primary components of its essential oils (EOs) are monoterpenoids, synthesized and stored in peltate glandular trichomes (PGTs). In general, the EO content in is relatively low. Methyl jasmonate (MJ) has been reported as an effective elicitor of terpenoid biosynthesis in medicinal plants, but the specific mechanisms underlying MJ's influence on remain unclear. In this study, exogenous application of MJ significantly increased the EO content, yield, and PGT density in a dose-dependent manner. At a 5 mM dose, the EO content and PGT density peaked, with increases of 71.20% and 53.69%, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that, in general, MJ treatment did not significantly alter the types or relative proportions of EO components of . However, L-menthol content decreased slightly by 7.90% under 5 mM MJ treatment. Transcriptome analysis identified 4,659 differentially expressed genes (DEGs) in MJ-treated leaves. KEGG enrichment analysis revealed that "Monoterpenoid biosynthesis" was among the most significantly enriched metabolic pathways. Key genes involved in jasmonic acid (JA) signaling ( and ) and monoterpenoid biosynthesis (, , , and ) were significantly up-regulated. Co-expression analysis, promoter binding element analysis and weighted gene co-expression network analysis (WGCNA) indicated that transcription factors (TFs) such as AP2/ERF, WRKY, MYB, and bHLH play crucial roles in regulating MJ-mediated monoterpenoid biosynthesis. Several key candidate TFs potentially involved in regulating monoterpenoid biosynthesis in were identified. These findings provide valuable insights into the molecular mechanisms regulating monoterpenoid accumulation in the genus.
唇萼薄荷是该属的一种芳香植物，因其药用和经济价值而闻名。其精油（EOs）的主要成分是单萜类化合物，在盾状腺毛（PGTs）中合成并储存。一般来说，唇萼薄荷中的EO含量相对较低。茉莉酸甲酯（MJ）已被报道为药用植物中萜类生物合成的有效诱导剂，但MJ对唇萼薄荷影响的具体机制仍不清楚。在本研究中，外源施用MJ以剂量依赖的方式显著提高了EO含量、产量和PGT密度。在5 mM剂量下，EO含量和PGT密度达到峰值，分别增加了71.20%和53.69%。气相色谱-质谱（GC-MS）分析表明，一般而言，MJ处理并未显著改变唇萼薄荷EO成分的类型或相对比例。然而，在5 mM MJ处理下，L-薄荷醇含量略有下降，降幅为7.90%。转录组分析在MJ处理的叶片中鉴定出4659个差异表达基因（DEGs）。KEGG富集分析表明，“单萜类生物合成”是最显著富集的代谢途径之一。参与茉莉酸（JA）信号传导（和）和单萜类生物合成（、、、和）的关键基因显著上调。共表达分析、启动子结合元件分析和加权基因共表达网络分析（WGCNA）表明，AP2/ERF、WRKY、MYB和bHLH等转录因子（TFs）在调节MJ介导的单萜类生物合成中起关键作用。鉴定出了几个可能参与调节唇萼薄荷单萜类生物合成的关键候选TFs。这些发现为调节该属单萜类积累的分子机制提供了有价值的见解。
