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噬菌体T4复制起点的位置。

Locations of bacteriophage T4 origins of replication.

作者信息

Yee J K, Marsh R C

出版信息

J Virol. 1985 May;54(2):271-7. doi: 10.1128/JVI.54.2.271-277.1985.

DOI:10.1128/JVI.54.2.271-277.1985
PMID:3989906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC254794/
Abstract

Partially replicated bacteriophage T4 DNA containing cytosine was isolated from cells 6.5 and 7 min after infection and cleaved with restriction endonuclease BglII or BamHI. Positions of replication eyes relative to the cleavage sites were observed by electron microscopy. Four groups of eyes were found. They are consistent with replication from origins located at map positions 34, 60, 73, and 86 kilobases. In individual molecules that contained two or three eyes, the distribution of the eyes agreed with the initiation of replication at more than one of these four assigned origins and possibly at two additional origins located near 15 and 110 kilobases, which were reported by P. M. Macdonald, R. M. Seaby, W. Brown, and G. Mosig (p. 111-116, in D. Schlessinger, ed., Microbiology--1983, 1983) and M. E. Halpern, T. Mattson, and A. W. Kozinski (Proc. Natl. Acad. Sci. U.S.A. 76:6137-6141, 1979).

摘要

感染后6.5分钟和7分钟从细胞中分离出含有胞嘧啶的部分复制的噬菌体T4 DNA，并用限制性内切酶BglII或BamHI切割。通过电子显微镜观察复制眼相对于切割位点的位置。发现了四组复制眼。它们与位于34、60、73和86千碱基图谱位置的起始点的复制一致。在含有两个或三个复制眼的单个分子中，复制眼的分布与在这四个指定起始点中的多个起始点以及可能在位于15和110千碱基附近的另外两个起始点处的复制起始一致，这两个起始点是由P.M.麦克唐纳、R.M.西比、W.布朗和G.莫西格报道的（见D.施莱辛格编，《微生物学——1983》，第111 - 116页，1983年）以及M.E.哈尔彭、T.马特森和A.W.科津斯基报道的（《美国国家科学院院刊》76:6137 - 6141，1979年）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f396/254794/541d3bf6a7d6/jvirol00122-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f396/254794/541d3bf6a7d6/jvirol00122-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f396/254794/541d3bf6a7d6/jvirol00122-0038-a.jpg

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Locations of bacteriophage T4 origins of replication.噬菌体T4复制起点的位置。
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引用本文的文献

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本文引用的文献

1
Early intermediates in bacteriophage T4 DNA replication and recombination.噬菌体T4 DNA复制与重组过程中的早期中间体
J Virol. 1983 Feb;45(2):813-31. doi: 10.1128/JVI.45.2.813-831.1983.
2
Identification of the origins of T4 DNA replication.T4噬菌体DNA复制起始点的鉴定。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7248-52. doi: 10.1073/pnas.79.23.7248.
3
Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI.噬菌体T4含胞嘧啶DNA上针对核酸内切酶BamHI、BglII、KpnI、PvuI、SalI和XbaI的限制酶切位点图谱。
两个新的早期噬菌体T4基因repEA和repEB，它们对于从E起始点开始的DNA复制很重要。
J Bacteriol. 1999 Nov;181(22):7115-25. doi: 10.1128/JB.181.22.7115-7125.1999.
4
Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4.噬菌体T4 58.3至65.5千碱基早期区域的核苷酸序列及分析
Nucleic Acids Res. 1986 Nov 11;14(21):8637-54. doi: 10.1093/nar/14.21.8637.
5
Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.某些噬菌体T4复制前基因的表达受损解释了大肠杆菌rho（nusD）突变体中T4 DNA合成受损的原因。
J Bacteriol. 1989 Jul;171(7):3872-80. doi: 10.1128/jb.171.7.3872-3880.1989.
6
Recombination hotspots in bacteriophage T4 are dependent on replication origins.噬菌体T4中的重组热点依赖于复制起点。
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6043-7. doi: 10.1073/pnas.88.14.6043.
7
Plasmid models for bacteriophage T4 DNA replication: requirements for fork proteins.噬菌体T4 DNA复制的质粒模型：对叉蛋白的要求。
J Virol. 1992 Dec;66(12):6960-8. doi: 10.1128/JVI.66.12.6960-6968.1992.
J Virol. 1981 Apr;38(1):104-14. doi: 10.1128/JVI.38.1.104-114.1981.
4
Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA.大肠杆菌产生的DNA的宿主特异性：影响DNA限制与修饰的细菌突变
J Mol Biol. 1966 Mar;16(1):118-33. doi: 10.1016/s0022-2836(66)80267-x.
5
The patterns of recombination and segregation in terminal regions of T4DNA molecules.T4 DNA 分子末端区域的重组和分离模式。
Mol Gen Genet. 1971;113(1):51-91. doi: 10.1007/BF00335007.
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