Huang Jingqiang, Wang Xianli, Ge Shengyang, Lu Xiao, Sun Chuanyu
Department of Urology Surgery, Huashan Hospital, Fudan University, No. 12, Middle Wulumuqi Road, Jing'an District, Shanghai, 200040 China.
School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025 China.
Cell Mol Bioeng. 2024 Oct 15;17(6):527-548. doi: 10.1007/s12195-024-00825-y. eCollection 2024 Dec.
Kidney cancer is a significant global health problem that affects nearly 1 in 25 of cancer patients. Prevalence, morbidity and mortality data associated with kidney cancer continue to increase every year, revealing a heavy economic and social burden. Organoid culture is a new research tool with great potential for many applications, particularly in cancer research. The integration of organoids with other emerging technologies has simultaneously expanded their potential applications. However, there is no thorough assessment of organoids in the field of renal cancer research.
This paper presents a comprehensive review of the current development of renal cancer organoids and discusses the corresponding solutions and future directions of renal cancer organoids.
In this study, we have compared the operating procedures of different organoid culture protocols and proposed a summary of constituents in culture media. Extensive discussions of renal cancer organoids, including generation and maintenance approaches, application scenarios, current challenges and prospects, have also been made. The information required for this study is extracted from literature databases such as PubMed, SCOPUS and Web of Science.
In this article, we systematically review thirteen successful methods for generating organoids to kidney cancer and provide practical guidelines for their construction as a reference. In addition, we also elucidate the clinical application of organoids, address the existing challenges and limitations, and highlight promising prospects.
Ultimately, we firmly believe that as kidney tumour organoids continue to develop and improve, they will become a crucial tool for treating kidney cancer.
肾癌是一个重大的全球健康问题，几乎每25名癌症患者中就有1人受其影响。与肾癌相关的患病率、发病率和死亡率数据每年都在持续上升，显示出沉重的经济和社会负担。类器官培养是一种具有巨大应用潜力的新研究工具，尤其在癌症研究中。类器官与其他新兴技术的整合同时扩展了它们的潜在应用。然而，在肾癌研究领域对类器官尚无全面评估。
本文对肾癌类器官的当前发展进行全面综述，并讨论肾癌类器官的相应解决方案和未来方向。
在本研究中，我们比较了不同类器官培养方案的操作程序，并对培养基中的成分进行了总结。还对肾癌类器官进行了广泛讨论，包括生成和维持方法、应用场景、当前挑战和前景。本研究所需信息从PubMed、SCOPUS和Web of Science等文献数据库中提取。
在本文中，我们系统地综述了13种成功生成肾癌类器官的方法，并为其构建提供实用指南以供参考。此外，我们还阐明了类器官的临床应用，解决了现有的挑战和局限性，并突出了有前景的前景。
最终，我们坚信随着肾肿瘤类器官不断发展和完善，它们将成为治疗肾癌的关键工具。
