School of Chemical and Material Engineering, Quzhou College, Quzhou 324000, China.
J Anal Methods Chem. 2013;2013:246986. doi: 10.1155/2013/246986. Epub 2013 Oct 27.
Gas chromatography-mass spectrometry and multivariate curve resolution were applied to the differential analysis of the volatile components in Agrimonia eupatoria specimens from different plant parts. After extracted with water distillation method, the volatile components in Agrimonia eupatoria from leaves and roots were detected by GC-MS. Then the qualitative and quantitative analysis of the volatile components in the main root of Agrimonia eupatoria was completed with the help of subwindow factor analysis resolving two-dimensional original data into mass spectra and chromatograms. 68 of 87 separated constituents in the total ion chromatogram of the volatile components were identified and quantified, accounting for about 87.03% of the total content. Then, the common peaks in leaf were extracted with orthogonal projection resolution method. Among the components determined, there were 52 components coexisting in the studied samples although the relative content of each component showed difference to some extent. The results showed a fair consistency in their GC-MS fingerprint. It was the first time to apply orthogonal projection method to compare different plant parts of Agrimonia eupatoria, and it reduced the burden of qualitative analysis as well as the subjectivity. The obtained results proved the combined approach powerful for the analysis of complex Agrimonia eupatoria samples. The developed method can be used to further study and quality control of Agrimonia eupatoria.
气相色谱-质谱联用技术和多变量曲线分辨法被应用于分析不同部位的龙芽草样本中的挥发性成分的差异。采用水蒸馏法提取后，利用 GC-MS 检测龙芽草叶和根中的挥发性成分。然后，借助子窗口因子分析将二维原始数据解析为质谱和色谱，完成龙芽草根中挥发性成分的定性和定量分析。从总离子色谱图中分离出 87 种成分中的 68 种，并进行了定量分析，占总含量的约 87.03%。然后，采用正交投影分辨法提取叶中的共有峰。在所确定的成分中，尽管各成分的相对含量在一定程度上存在差异，但研究样品中仍存在 52 种共存成分。结果表明，GC-MS 指纹图谱具有良好的一致性。这是首次将正交投影法应用于龙芽草不同部位的比较，减少了定性分析的负担和主观性。所得结果证明，该联合方法对于分析复杂的龙芽草样品非常有效。该方法可用于进一步研究和控制龙芽草的质量。
