Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, Poland.
Department of Clinical Chemistry and Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland.
Cells. 2022 Oct 15;11(20):3238. doi: 10.3390/cells11203238.
The promotion of biologically based treatment strategies in restorative dentistry is of paramount importance, as invasive treatments should be avoided to maintain the tooth's vitality. This study aimed to assess the biocompatibility of commercially available bioactive materials that can be used for dental pulp capping. The study was performed with a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on the following six specific bioactive materials: ProRoot MTA (Dentsply Sirona), MTA Angelus (Angelus), Biodentine (Septodont), TheraCal LC (Bisco), ACTIVA BioACTIVE (Pulpdent) and Predicta Bioactive Bulk (Parkell). The cytotoxicity of the investigated agents was measured using a resazurin-based cell viability assay, while the genotoxicity was evaluated using an alkaline comet assay. Additionally, flow cytometry (FC) apoptosis detection was conducted with a FITC (fluorescein isothiocyanate) Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was carried out with propidium iodide staining. The results of this study showed no significant cytotoxicity and genotoxicity ( > 0.05) in ProRoot MTA, MTA Angelus, Biodentine, ACTIVA BioACTIVE and Predicta Bioactive. Conversely, TheraCal LC presented a significant decrease ( < 0.001). In conclusion, due to excellent biocompatibility and low cytotoxicity, MTA, Biodentine, ACTIVA BioACTIVE and Predicta Bioactive may be suitable for pulp capping treatments. On the other hand, due to the high cytotoxicity of TheraCal LC, its use should be avoided in vital pulp therapies.
在修复牙科中推广基于生物学的治疗策略至关重要，因为应避免侵袭性治疗以保持牙齿活力。本研究旨在评估可用于牙髓盖髓的市售生物活性材料的生物相容性。该研究使用单核细胞/巨噬细胞外周血 SC 细胞系（ATCC CRL-9855）在以下六种特定的生物活性材料上进行：ProRoot MTA（登士柏西诺德）、MTA Angelus（Angelus）、Biodentine（思博安）、TheraCal LC（碧兰）、ACTIVA BioACTIVE（派丽登）和 Predicta Bioactive Bulk（派力登）。使用基于 Resazurin 的细胞活力测定法测量了研究试剂的细胞毒性，而使用碱性彗星试验评估了遗传毒性。此外，使用 FITC（异硫氰酸荧光素）Annexin V Apoptosis Detection Kit I 通过流式细胞术（FC）进行凋亡检测。通过碘化丙啶染色进行 FC 细胞周期阻滞评估。本研究的结果表明，ProRoot MTA、MTA Angelus、Biodentine、ACTIVA BioACTIVE 和 Predicta Bioactive 中没有明显的细胞毒性和遗传毒性（>0.05）。相反，TheraCal LC 表现出显著降低（<0.001）。总之，由于具有优异的生物相容性和低细胞毒性，MTA、Biodentine、ACTIVA BioACTIVE 和 Predicta Bioactive 可能适用于牙髓盖髓治疗。另一方面，由于 TheraCal LC 的细胞毒性高，应避免在活髓治疗中使用。
