Chen Shenghua, Chen Mingheng, Han Yuyang, Chen Zhiquan, Mu Xu, He Chunjing, Zhao Jing, Zhang Lin, Huang Zhifeng
Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, China.
Department of Burns and Wound Repair Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.
Arch Dermatol Res. 2025 Feb 22;317(1):451. doi: 10.1007/s00403-025-03913-9.
This study explored the effects of Moist Exposed Burn Ointment (MEBO) on the healing process of pressure-induced wounds. Using a stage IV pressure ulcer model established in 42 rats, divided equally into a control gel group and an MEBO group, we investigated the efficacy of MEBO through topical application. The control group received Carbomer gel, while the MEBO group was treated with MEBO until complete wound healing. Results showed that MEBO significantly accelerated wound healing compared to the control group. Histological analysis, including hematoxylin and eosin (HE) staining and Masson's trichrome staining, revealed enhanced epithelialization and collagen deposition in the MEBO group. Furthermore, 16S rRNA sequencing indicated that MEBO reduced microbial diversity at the wound site and reshaped the microbial composition. Notably, The increased abundance of Acinetobacter and Staphylococcus, coupled with a reduction in Pseudomonas, may reflect a shift in the wound microbiome that could be conducive to healing. However, the exact role of these microbial shifts in promoting wound healing requires further investigation, as microbial dynamics in wound environments are complex and context-dependent. These findings suggest that MEBO facilitates wound healing by optimizing the wound microbiome, thereby offering a promising therapeutic approach for managing pressure ulcers.
本研究探讨了湿润烧伤膏（MEBO）对压力性伤口愈合过程的影响。我们使用在42只大鼠中建立的IV期压疮模型，将其平均分为对照凝胶组和MEBO组，通过局部应用来研究MEBO的疗效。对照组接受卡波姆凝胶，而MEBO组用MEBO治疗直至伤口完全愈合。结果表明，与对照组相比，MEBO显著加速了伤口愈合。组织学分析，包括苏木精和伊红（HE）染色及Masson三色染色，显示MEBO组上皮化和胶原沉积增强。此外，16S rRNA测序表明，MEBO降低了伤口部位的微生物多样性并重塑了微生物组成。值得注意的是，不动杆菌和葡萄球菌丰度增加，同时假单胞菌减少，这可能反映了伤口微生物群的转变，有利于愈合。然而，这些微生物变化在促进伤口愈合中的确切作用需要进一步研究，因为伤口环境中的微生物动态是复杂且依赖于背景的。这些发现表明，MEBO通过优化伤口微生物群促进伤口愈合，从而为治疗压疮提供了一种有前景的治疗方法。
