Khalid Waseem, Benmebarek Imed E, Zargarchi Sina, Kumar Prashant, Javed Miral, Moreno Andres, Sharma Aanchal, Nayik Gulzar Ahmad, Esatbeyoglu Tuba
Department of Organic Chemistry, Faculty of Chemical Sciences and Technologies, University of Castilla La Mancha, 13071 Ciudad Real, Spain; Department of Molecular Food Chemistry and Food Development, Institute of Food and One Health, Gottfried Wilhelm Leibniz University Hannover, Am Kleinen Felde 30, 30167 Hannover, Germany.
Department of Organic Chemistry, Faculty of Chemical Sciences and Technologies, University of Castilla La Mancha, 13071 Ciudad Real, Spain.
Ultrason Sonochem. 2025 Mar;114:107276. doi: 10.1016/j.ultsonch.2025.107276. Epub 2025 Feb 22.
The chickpea (Cicer arietinum L.) root is an agricultural by-product with the potential for extracting valuable bioactive compounds that often remains underutilized. This study introduces an integrated extraction methodology to enhance the extraction of bioactives using atmospheric air low-pressure cold plasma (CP) treatment followed by ultrasound-assisted extraction (UAE) with natural deep eutectic solvents (NADES). Chickpea root powder was first subjected to CP treatment under optimized conditions (power, pressure, and time) identified via response surface methodology (RSM). Subsequently, UAE-NADES extraction was performed to maximize the results of antioxidant activity (DPPH) and total phenolic content (TPC). The integrated CP-UAE-NADES process enhanced TPC and DPPH compared to the untreated sample (non-CP). The optimum conditions were 11.5 min, 52 W, and 0.65 mbar. The predicted values of the Box-Behnken design for TPC and DPPH were compatible with the experimental Furthermore, microbial load reduction and color stability were analyzed to ensure chickpea root quality and functionality. The combined extraction methodology offers a sustainable and eco-friendly approach for the valorization of chickpea root as a source of bioactives, with potential applications in functional foods, nutraceuticals, and pharmaceuticals.
鹰嘴豆（Cicer arietinum L.）根是一种农业副产品，具有提取有价值生物活性化合物的潜力，但往往未得到充分利用。本研究引入了一种综合提取方法，通过常压空气低压冷等离子体（CP）处理，随后用天然深共熔溶剂（NADES）进行超声辅助提取（UAE），以提高生物活性成分的提取率。鹰嘴豆根粉首先在通过响应面法（RSM）确定的优化条件（功率、压力和时间）下进行CP处理。随后，进行UAE-NADES提取，以最大化抗氧化活性（DPPH）和总酚含量（TPC）的结果。与未处理的样品（非CP）相比，CP-UAE-NADES综合工艺提高了TPC和DPPH。最佳条件为11.5分钟、52瓦和0.65毫巴。Box-Behnken设计对TPC和DPPH的预测值与实验值相符。此外，还分析了微生物负荷降低和颜色稳定性，以确保鹰嘴豆根的质量和功能。这种联合提取方法为将鹰嘴豆根作为生物活性成分来源进行增值提供了一种可持续且环保的方法，在功能性食品、营养保健品和制药领域具有潜在应用。
