Poloni Valeria L, Pérez María Eugenia, Escobar Franco, Luna Julieta, Pereyra Yanina, Cristofolini Andrea, Magnoli Alejandra, Cavaglieri Lilia
Departamento de Microbiología E Inmunología, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, (5800), Río Cuarto, Cordoba, Argentina.
Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.
Probiotics Antimicrob Proteins. 2025 Feb 25. doi: 10.1007/s12602-025-10492-8.
The objective of this work was to obtain postbiotics derived from the cell wall of Saccharomyces cerevisiae RC016 by applying different strategies and to characterize them in terms of their antitoxin capacity in vitro and their in vivo impact on intestinal integrity, evaluating the modulation of the microbiota and the histomorphometry of the intestine. Moreover, the impact of dried strategies on chemical groups related to food toxin adsorption was analyzed. Nine mechanical and enzymatic cell disruption treatments were assayed using S. cerevisiae RC016 biomass to obtain the postbiotic under study. Then, postbiotics were characterized using high-resolution optical microscopy and assayed for in vitro studies related to their antitoxin activity (adsorption and degradation of aflatoxin B). Postbiotics were dried using freeze-dried and spray-dried methods and subjected to FT-IR spectroscopy. Finally, the postbiotic efficacy was determined on an in vivo study conducted on 16 male and female BALB/c mice, divided into two experimental groups, each experimental group (n = 8) separated by sex in different cages (four females and four males): untreated (control) and yeast wall treated (YW); (a) female control; (b) male control; (c) female control + YW; (d) male control + YW. The intestinal microbiota showed significant differences in the counts of LAB and enterobacteria between male and female animals. The histomorphometric analysis showed a significant increase in villi height and width, as well as crypt depth, compared to the control group in male mice with the addition of the postbiotic solution of S. cerevisiae. These findings open new avenues for further optimizing postbiotics' production processes and evaluating their efficacy across diverse conditions and populations.
这项工作的目的是通过应用不同策略从酿酒酵母RC016的细胞壁中获得后生元，并在体外抗毒素能力以及它们对肠道完整性的体内影响方面对其进行表征，评估微生物群的调节和肠道的组织形态计量学。此外，还分析了干燥策略对与食品毒素吸附相关的化学基团的影响。使用酿酒酵母RC016生物质对九种机械和酶促细胞破碎处理进行了测定，以获得所研究的后生元。然后，使用高分辨率光学显微镜对后生元进行表征，并对其与抗毒素活性相关的体外研究（黄曲霉毒素B的吸附和降解）进行测定。后生元采用冷冻干燥和喷雾干燥方法进行干燥，并进行傅里叶变换红外光谱分析。最后，在对16只雄性和雌性BALB/c小鼠进行的体内研究中确定后生元的功效，将其分为两个实验组，每个实验组（n = 8）按性别分在不同笼子中（四只雌性和四只雄性）：未处理（对照）和酵母壁处理（YW）；（a）雌性对照；（b）雄性对照；（c）雌性对照 + YW；（d）雄性对照 + YW。肠道微生物群在雄性和雌性动物之间的乳酸菌和肠杆菌计数上显示出显著差异。组织形态计量学分析表明，与添加酿酒酵母后生元溶液的雄性小鼠对照组相比，绒毛高度、宽度以及隐窝深度均显著增加。这些发现为进一步优化后生元的生产工艺以及评估其在不同条件和人群中的功效开辟了新途径。
