Tanfloc修饰的钛表面：优化血液凝固活性和干细胞相容性。
Tanfloc-Modified Titanium Surfaces: Optimizing Blood Coagulant Activity and Stem Cell Compatibility.
作者信息
Singh Ramesh, Madruga Liszt Y C, Savargaonkar Aniruddha, Martins Alessandro F, Kipper Matt J, Popat Ketul C
机构信息
Department of Bioengineering, College of Engineering and Computing, George Mason University, Fairfax, Virginia 22030, United States.
Department of Mechanical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
出版信息
ACS Biomater Sci Eng. 2025 Mar 10;11(3):1445-1455. doi: 10.1021/acsbiomaterials.4c02106. Epub 2025 Feb 27.
This study explores the synergistic effects of combining titania nanotubes (TiNTs) with the biopolymer Tanfloc (TAN) to enhance the surface properties of TiNTs for biomedical applications. We investigated the interactions of blood components and human adipose-derived stem cells (ADSCs) with TiNT surfaces covalently functionalized with Tanfloc (TAN), an aminolyzed polyphenolic tannin derivative. The functionalized surfaces (TiNT-TAN) have great potential to control protein adsorption and platelet adhesion and activation. Fluorescence and scanning electron microscopy (SEM) were used to analyze platelet adherence and activation. The amphoteric nature and multiple functional groups on TAN can control blood protein adsorption, platelet adhesion, and activation. Further, the modified surface supports adipose-derived stem cell (ADSC) viability, attachment, and growth without any cytotoxic effect. The TAN conjugation significantly (**** < 0.0001) increased the proliferation rate of ADSCs compared to the TiNT surfaces.
本研究探讨了将二氧化钛纳米管（TiNTs）与生物聚合物Tanfloc（TAN）结合的协同效应，以增强TiNTs在生物医学应用中的表面性能。我们研究了血液成分和人脂肪来源干细胞（ADSCs）与用Tanfloc（TAN）共价功能化的TiNT表面之间的相互作用，Tanfloc是一种氨基化的多酚单宁衍生物。功能化表面（TiNT-TAN）在控制蛋白质吸附、血小板粘附和激活方面具有巨大潜力。利用荧光和扫描电子显微镜（SEM）分析血小板的粘附和激活情况。TAN的两性性质和多个官能团可以控制血液蛋白质吸附、血小板粘附和激活。此外，改性表面支持脂肪来源干细胞（ADSC）的活力、附着和生长，且无任何细胞毒性作用。与TiNT表面相比，TAN共轭显著（****<0.0001）提高了ADSCs的增殖率。
Zotero 插件