使用乳酸分析仪LA 640测定乳酸：一项批判性研究。

Lactate determination with the lactate analyser LA 640: a critical study.

作者信息

Geyssant A, Dormois D, Barthelemy J C, Lacour J R

出版信息

Scand J Clin Lab Invest. 1985 Apr;45(2):145-9. doi: 10.3109/00365518509160987.

Abstract

Using the new method of sample preparation developed by the manufacturer in 1979, the lactate analyser LA 640 now provides reliable measurements of blood lactic acid from blood micro-samples of 20 microliters within 2 min. Reproducibility is comparable to that obtained by spectrophotometric assay with +/- 0.2 mmol . l-1 for 95% confidence limits. High accuracy was also obtained with 95% linked confidence limits within +/- 0.19 mmol . l-1 and +/- 0.46 mmol . l-1 for blood lactate concentrations of from 2-4 and 7-9 mmol . l-1 respectively. Due to a lactate concentration increase of 0.5 mmol . l-1 . h-1 in whole blood kept at 20 degrees C, the blood must be immediately diluted in the buffer haemolysing solution after withdrawal. This diluted sample can then be stored at room temperature without alteration, provided the concentration in the dilution is lower than 0.8 mmol . l-1. For serial successive measurements, a new sample can be tested before the electrode current reaches its background value.

摘要

采用制造商于1979年研发的新样本制备方法，乳酸分析仪LA 640现在能够在2分钟内对20微升的血液微量样本中的血乳酸进行可靠测量。其重现性与分光光度法相当，95%置信限为±0.2 mmol·l⁻¹。对于血乳酸浓度分别为2 - 4 mmol·l⁻¹和7 - 9 mmol·l⁻¹的情况，也获得了高精度，95%关联置信限分别为±0.19 mmol·l⁻¹和±0.46 mmol·l⁻¹。由于在20℃保存的全血中乳酸浓度以0.5 mmol·l⁻¹·h⁻¹的速度增加，采血后必须立即将血液在缓冲溶血溶液中稀释。如果稀释后的浓度低于0.8 mmol·l⁻¹，那么该稀释样本可以在室温下保存而不变质。对于连续测量，在电极电流达到其背景值之前可以测试新样本。