Sonnenfeld G, Griffith R B, Hudgens R W
Arch Toxicol. 1985 Dec;58(2):120-2. doi: 10.1007/BF00348322.
A system for the simultaneous exposure of monolayer cell cultures to mainstream (MS) and sidestream (SS) smoke from the same cigarette was utilized to study the effects of smoke generation and manipulation variables on the cytotoxicity of smoke to monolayer cultures of mouse fibroblast-like L-929 cells. The cytotoxicity of MS smoke was decreased with increasing smoke age (up to 8.7 s), smoke dilution, and the quantity of activated charcoal in filters. Acetate filters had little effect on cell mortality, and the age-of-smoke effect was not evident for MS smoke generated with a low puff volume and rapid dilution. The cytotoxicity of SS smoke also decreased rapidly with increasing smoke age and dilution. The results indicate that the gas phase of smoke may be of major importance in generating the observed toxic effects. These results may be of potential future significance in defining the requirements for a less toxic cigarette, in considering the hazards of SS smoke, and in evaluating in vivo inhalation studies.
一种用于将单层细胞培养物同时暴露于同一支香烟的主流（MS）烟雾和侧流（SS）烟雾的系统，被用于研究烟雾产生和操作变量对烟雾对小鼠成纤维样L-929细胞单层培养物细胞毒性的影响。随着烟雾龄（长达8.7秒）增加、烟雾稀释以及过滤嘴中活性炭量增加，主流烟雾的细胞毒性降低。醋酸纤维过滤嘴对细胞死亡率影响很小，对于低抽吸量和快速稀释产生的主流烟雾，烟雾龄效应不明显。侧流烟雾的细胞毒性也随着烟雾龄和稀释度增加而迅速降低。结果表明，烟雾的气相在产生观察到的毒性作用中可能起主要作用。这些结果在确定低毒性香烟的要求、考虑侧流烟雾的危害以及评估体内吸入研究方面可能具有潜在的未来意义。
