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Simple screening method for identification of nonpleiotropic mutants for exoenzyme production.

作者信息

Rose K E, Heath H E, Sloan G L

出版信息

Appl Environ Microbiol. 1985 May;49(5):1335-7. doi: 10.1128/aem.49.5.1335-1337.1985.

DOI:10.1128/aem.49.5.1335-1337.1985
PMID:4004249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238553/
Abstract

A differential medium that distinguishes between pleiotropic and nonpleiotropic mutants for exoenzyme production has been developed for Staphylococcus simulans biovar staphylolyticus. The medium will facilitate genetic analysis of exoenzyme production by this organism. Generally useful strategies for increasing the sensitivity of indicator plates for detection of exoenzyme activities are presented.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df9c/238553/ac575cc44b42/aem00150-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df9c/238553/ac575cc44b42/aem00150-0320-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df9c/238553/ac575cc44b42/aem00150-0320-a.jpg

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Simple screening method for identification of nonpleiotropic mutants for exoenzyme production.
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本文引用的文献

1
Typing of coagulase-positive staphylococci by proteolytic activity on buffered caseinate-agar, with special reference to bacteriophage nontypable strains.缓冲酪蛋白琼脂上蛋白水解活性对凝固酶阳性葡萄球菌的分型，特别是对非噬菌体定型菌株的研究。
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Chitinase-overproducing mutant of Serratia marcescens.粘质沙雷氏菌几丁质酶高产突变体。
Appl Environ Microbiol. 1981 Mar;41(3):664-9. doi: 10.1128/aem.41.3.664-669.1981.
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The characteristics of extracellular protein secretion by Staphylococcus staphylolyticus.
J Gen Microbiol. 1980 Jun;118(2):529-33. doi: 10.1099/00221287-118-2-529.
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Intracellular accumulation of extracellular proteins by pleiotropic export mutants of Aeromonas hydrophila.嗜水气单胞菌多效性输出突变体对细胞外蛋白质的细胞内积累
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5
Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.铜绿假单胞菌的磷脂酶C调节突变，导致几种磷酸盐阻遏蛋白的组成型合成。
J Bacteriol. 1982 Jun;150(3):1221-6. doi: 10.1128/jb.150.3.1221-1226.1982.
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Coordinate production of three exoenzymes of Staphylococcus staphylolyticus.葡萄球菌溶菌素产生的三种胞外酶的协同产生。
J Gen Microbiol. 1982 Jul;128(7):1529-35. doi: 10.1099/00221287-128-7-1529.
7
Isolation of mutants of Staphylococcus aureus lacking extracellular nuclease activity.缺乏细胞外核酸酶活性的金黄色葡萄球菌突变体的分离
J Bacteriol. 1970 Mar;101(3):921-4. doi: 10.1128/jb.101.3.921-924.1970.
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Bacteriolytic enzymes from staphylococci.来自葡萄球菌的溶菌酶
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9
Membrane mutation related to the production of extracellular -amylase and protease in bacillus subtilis.与枯草芽孢杆菌胞外淀粉酶和蛋白酶产生相关的膜突变
Biochem Biophys Res Commun. 1973 Feb 5;50(3):765-70. doi: 10.1016/0006-291x(73)91310-7.
10
Pleiotropic mutations in Serratia marcescens which increase the synthesis of certain exocellular proteins and the rate of spontaneous prophage induction.粘质沙雷氏菌中的多效性突变，可增加某些胞外蛋白的合成以及原噬菌体自发诱导的速率。
Mol Gen Genet. 1973 Aug 17;124(3):197-206. doi: 10.1007/BF00293091.