• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

VI 型分泌系统相关 FHA 结构域蛋白 TagH 调节霍乱弧菌的溶血活性和毒力。

Type VI secretion system-associated FHA domain protein TagH regulates the hemolytic activity and virulence of Vibrio cholerae.

机构信息

Department of Laboratory Medicine, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.

School of Laboratory Medicine, Zunyi Medical University, Zunyi, Guizhou, China.

出版信息

Gut Microbes. 2022 Jan-Dec;14(1):2055440. doi: 10.1080/19490976.2022.2055440.

DOI:10.1080/19490976.2022.2055440
PMID:35383540
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8993066/
Abstract

The type VI secretion system (T6SS) and hemolysin HlyA are important virulence factors in . The forkhead-associated (FHA) domain is a conserved phosphopeptide binding domain that exists in many regulatory modules. The FHA domain protein-encoding gene is conserved in the T6SS gene cluster and regulates the assembly and secretion of the T6SS. This study shows for the first time that the FHA domain protein TagH plays a role in controlling the hemolytic activity of , in addition to regulating the T6SS. TagH negatively regulates HlyA expression at the transcriptional and post-translational levels. The phosphopeptide binding sites of the FHA domain of TagH play a key role in the regulation of hemolytic activity. The deletion of enhances the intestinal pathogenicity and extraintestinal invasion ability of , which mainly depend on the expression of HlyA. This study provides evidence that helps unravel the novel regulatory role of TagH in HlyA and provides critical insights which will aid in the development of strategies to manage HlyA.

摘要

VI 型分泌系统（T6SS）和溶血素 HlyA 是 重要的毒力因子。FHA 结构域是一个保守的磷酸肽结合结构域，存在于许多调节模块中。FHA 结构域蛋白编码基因在 T6SS 基因簇中保守，调节 T6SS 的组装和分泌。这项研究首次表明，FHA 结构域蛋白 TagH 除了调节 T6SS 外，还在控制 的溶血活性中发挥作用。TagH 在转录和翻译后水平上负调控 HlyA 的表达。TagH FHA 结构域的磷酸肽结合位点在调节溶血活性中起关键作用。缺失 增强了 的肠道致病性和肠外侵袭能力，这主要依赖于 HlyA 的表达。这项研究提供了证据，有助于揭示 TagH 在 HlyA 中的新调控作用，并为开发管理 HlyA 的策略提供了重要的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/a2e7c87147e4/KGMI_A_2055440_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/30e12929c886/KGMI_A_2055440_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/70372da891a1/KGMI_A_2055440_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/1e24d7d377c7/KGMI_A_2055440_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/156e68797255/KGMI_A_2055440_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/92d0b906935d/KGMI_A_2055440_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/1f970c56aee2/KGMI_A_2055440_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/3898aded4e81/KGMI_A_2055440_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/e94623abe4bb/KGMI_A_2055440_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/a2e7c87147e4/KGMI_A_2055440_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/30e12929c886/KGMI_A_2055440_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/70372da891a1/KGMI_A_2055440_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/1e24d7d377c7/KGMI_A_2055440_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/156e68797255/KGMI_A_2055440_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/92d0b906935d/KGMI_A_2055440_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/1f970c56aee2/KGMI_A_2055440_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/3898aded4e81/KGMI_A_2055440_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/e94623abe4bb/KGMI_A_2055440_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/345c/8993066/a2e7c87147e4/KGMI_A_2055440_F0009_OC.jpg

相似文献

1
Type VI secretion system-associated FHA domain protein TagH regulates the hemolytic activity and virulence of Vibrio cholerae.VI 型分泌系统相关 FHA 结构域蛋白 TagH 调节霍乱弧菌的溶血活性和毒力。
Gut Microbes. 2022 Jan-Dec;14(1):2055440. doi: 10.1080/19490976.2022.2055440.
2
Type VI Secretion Systems: Environmental and Intra-host Competition of Vibrio cholerae.VI 型分泌系统：霍乱弧菌的环境和宿主内竞争。
Adv Exp Med Biol. 2023;1404:41-63. doi: 10.1007/978-3-031-22997-8_3.
3
Pesticin-Like Effector VgrG3 Targeting Peptidoglycan Delivered by the Type VI Secretion System Contributes to Vibrio cholerae Interbacterial Competition.类杀虫剂效应物 VgrG3 靶向由 VI 型分泌系统输送的肽聚糖，有助于霍乱弧菌的细菌间竞争。
Microbiol Spectr. 2023 Feb 14;11(1):e0426722. doi: 10.1128/spectrum.04267-22. Epub 2023 Jan 10.
4
Bile Salts Modulate the Mucin-Activated Type VI Secretion System of Pandemic Vibrio cholerae.胆汁盐调节大流行霍乱弧菌的粘蛋白激活VI型分泌系统。
PLoS Negl Trop Dis. 2015 Aug 28;9(8):e0004031. doi: 10.1371/journal.pntd.0004031. eCollection 2015.
5
Secretome analysis of Vibrio cholerae type VI secretion system reveals a new effector-immunity pair.霍乱弧菌VI型分泌系统的分泌蛋白组分析揭示了一对新的效应蛋白-免疫蛋白组合。
mBio. 2015 Mar 10;6(2):e00075. doi: 10.1128/mBio.00075-15.
6
Vibrio cholerae Type VI Activity Alters Motility Behavior in Mucin.霍乱弧菌 VI 型分泌系统活性改变粘蛋白中的运动行为。
J Bacteriol. 2020 Nov 19;202(24). doi: 10.1128/JB.00261-20.
7
TagF-mediated repression of bacterial type VI secretion systems involves a direct interaction with the cytoplasmic protein Fha.TagF 介导的细菌 VI 型分泌系统的抑制作用涉及与细胞质蛋白 Fha 的直接相互作用。
J Biol Chem. 2018 Jun 8;293(23):8829-8842. doi: 10.1074/jbc.RA117.001618. Epub 2018 Mar 29.
8
Sensing of intracellular Hcp levels controls T6SS expression in .胞内 Hcp 水平的感应控制. 中的 T6SS 表达。
Proc Natl Acad Sci U S A. 2021 Jun 22;118(25). doi: 10.1073/pnas.2104813118.
9
Sporadic type VI secretion in seventh pandemic .第七次大流行中的散发性六型分泌系统。
Microbiology (Reading). 2023 May;169(5). doi: 10.1099/mic.0.001329.
10
c-di-GMP inhibits LonA-dependent proteolysis of TfoY in Vibrio cholerae.c-di-GMP 抑制霍乱弧菌 LonA 依赖性 TfoY 的蛋白水解。
PLoS Genet. 2020 Jun 26;16(6):e1008897. doi: 10.1371/journal.pgen.1008897. eCollection 2020 Jun.

引用本文的文献

1
Dynamic and intricate regulation by the Csr sRNAs in the Arctic Pseudoalteromonas fuliginea.北极假交替单胞菌中Csr小RNA的动态复杂调控
Commun Biol. 2025 Mar 5;8(1):369. doi: 10.1038/s42003-025-07780-y.
2
Improvement of the Antioxidant and Antitumor Activities of Benzimidazole-Chitosan Quaternary Ammonium Salt on Drug Delivery Nanogels.苯并咪唑壳聚糖季铵盐载药纳米凝胶的抗氧化和抗肿瘤活性的改善。
Mar Drugs. 2024 Jan 11;22(1):40. doi: 10.3390/md22010040.
3
DegS protease regulates antioxidant capacity and adaptability to oxidative stress environment in .

本文引用的文献

1
Crosstalks Between Gut Microbiota and .肠道微生物群与. 的串扰。
Front Cell Infect Microbiol. 2020 Oct 23;10:582554. doi: 10.3389/fcimb.2020.582554. eCollection 2020.
2
TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae.TssA-TssM-TagA 相互作用调节霍乱弧菌的 VI 型分泌系统鞘管组装。
Nat Commun. 2020 Oct 8;11(1):5065. doi: 10.1038/s41467-020-18807-9.
3
Phenotypic and Genotypic Properties of non-O1, non-O139 Isolates Recovered from Domestic Ducks in Germany.从德国家鸭中分离出的非O1、非O139菌株的表型和基因型特性
DegS 蛋白酶调节 抗氧化能力和对氧化应激环境的适应能力。
Front Cell Infect Microbiol. 2023 Nov 20;13:1290508. doi: 10.3389/fcimb.2023.1290508. eCollection 2023.
4
Comparative secretomic and proteomic analysis reveal multiple defensive strategies developed by against the heavy metal (Cd, Ni, Pb, and Zn) stresses.比较分泌蛋白质组学和蛋白质组学分析揭示了[具体对象未给出]针对重金属（镉、镍、铅和锌）胁迫所形成的多种防御策略。
Front Microbiol. 2023 Oct 26;14:1294177. doi: 10.3389/fmicb.2023.1294177. eCollection 2023.
5
Whole Genome Analysis of a Non-O1, Non-O139 Detected from Human Blood in China.在中国从人血液中检测到的一株非O1、非O139型霍乱弧菌的全基因组分析
Infect Drug Resist. 2023 Aug 21;16:5453-5461. doi: 10.2147/IDR.S420095. eCollection 2023.
6
Regulation of type VI secretion systems at the transcriptional, posttranscriptional and posttranslational level.VI 型分泌系统在转录、转录后和翻译后水平的调控。
Microbiology (Reading). 2023 Aug;169(8). doi: 10.1099/mic.0.001376.
7
The FHA domain protein ArnA functions as a global DNA damage response repressor in the hyperthermophilic archaeon .FHA 结构域蛋白 ArnA 在高温古菌中作为全局 DNA 损伤反应的抑制剂发挥作用。
mBio. 2023 Aug 31;14(4):e0094223. doi: 10.1128/mbio.00942-23. Epub 2023 Jun 30.
8
Unveiling a Virulence-Regulating Mechanism in Aeromonas hydrophila: a Quantitative Exoproteomic Analysis of an AraC-Like Protein.揭示嗜水气单胞菌毒力调控机制：AraC 样蛋白的定量外蛋白质组学分析。
Front Immunol. 2023 May 9;14:1191209. doi: 10.3389/fimmu.2023.1191209. eCollection 2023.
9
DegS protease regulates the motility, chemotaxis, and colonization of .DegS蛋白酶调节……的运动性、趋化性和定殖。 （原文此处不完整，缺少具体所指对象）
Front Microbiol. 2023 Apr 5;14:1159986. doi: 10.3389/fmicb.2023.1159986. eCollection 2023.
10
MlrA, a MerR family regulator in , senses the anaerobic signal in the small intestine of the host to promote bacterial intestinal colonization.MlrA，一种在 中存在的 MerR 家族调节剂，感知宿主小肠内的厌氧信号，从而促进细菌的肠道定植。
Gut Microbes. 2022 Jan-Dec;14(1):2143216. doi: 10.1080/19490976.2022.2143216.
Microorganisms. 2020 Jul 23;8(8):1104. doi: 10.3390/microorganisms8081104.
4
NGO2105 Is an Autotransporter Protein Involved in Adhesion to Human Cervical Epithelial Cells and Colonization.NGO2105是一种自转运蛋白，参与对人宫颈上皮细胞的黏附及定植。
Front Microbiol. 2020 Jun 25;11:1395. doi: 10.3389/fmicb.2020.01395. eCollection 2020.
5
Liver toxicity of macrolide antibiotics in zebrafish.大环内酯类抗生素在斑马鱼中的肝毒性。
Toxicology. 2020 Aug;441:152501. doi: 10.1016/j.tox.2020.152501. Epub 2020 May 23.
6
Cholera Toxin Production in O1 El Tor Biotype Strains in Single-Phase Culture.单相培养中O1埃尔托生物型菌株霍乱毒素的产生
Front Microbiol. 2020 May 5;11:825. doi: 10.3389/fmicb.2020.00825. eCollection 2020.
7
Upregulation of virulence genes promotes biofilm hyperinfectivity.毒力基因的上调促进生物膜的超强感染性。
Proc Natl Acad Sci U S A. 2020 May 19;117(20):11010-11017. doi: 10.1073/pnas.1916571117. Epub 2020 Apr 30.
8
Non-O1, Non-O139 (NOVC) Bacteremia: Case Report and Literature Review, 2015-2019.非O1、非O139（NOVC）菌血症：病例报告及文献综述，2015 - 2019年
Infect Drug Resist. 2020 Apr 7;13:1009-1016. doi: 10.2147/IDR.S245806. eCollection 2020.
9
Genomic characterization of the non-O1/non-O139 strain that caused a gastroenteritis outbreak in Santiago, Chile, 2018.2018 年智利圣地亚哥一起胃肠炎暴发疫情中 O 不凝集性非 O1/O139 血清型菌株的基因组特征。
Microb Genom. 2020 Mar;6(3). doi: 10.1099/mgen.0.000340.
10
Exploration of the effects of a mutant on the growth of and the global regulatory function of by RNA sequencing.通过RNA测序探索一种突变体对[具体对象1]生长及[具体对象2]全局调控功能的影响。
PeerJ. 2019 Oct 23;7:e7959. doi: 10.7717/peerj.7959. eCollection 2019.