Sabeti Sara, Koosha Mona, Kazemirad Elham, Mirabedini Zahra, Mohebali Mehdi, Saberi Reza, Fakhar Mahdi, Hajjaran Homa
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
BMC Infect Dis. 2025 Mar 6;25(1):319. doi: 10.1186/s12879-025-10717-9.
Leishmaniasis is a parasitic disease caused by an intracellular protozoan, Leishmania. Various factors, including host immunity and the Leishmania species, influence the manifestation and severity of the disease. Recent investigations have shed light on the potentially significant role of Leishmania RNA virus (LRV) in the clinical prognosis of leishmaniasis. This study aims to investigate the influence of LRV2 + on various pathogenic genes of Leishmania.
In this study, 35 Leishmania isolates were obtained from patients diagnosed with cutaneous leishmaniasis (CL). Leishmania species and the presence of LRV2 + were identified with the PCR-RFLP and semi-nested PCR methods, respectively. Additionally, the RNA expression levels of cysteine protease (CP), heat shock protein 70 (HSP70), heat shock protein 83 (HSP83), glycoprotein 63 (GP63), and mannose phosphate isomerase (MPI) were assessed in LRV2 + and LRV2- Leishmania clinical isolates using RT-qPCR.
Out of the 35 isolates, 20 were selected from CL patients, all confirmed as Leishmania major. These isolates were divided into two groups, LRV2 + and LRV2-, with 10 isolates in each group. RT-qPCR analysis revealed that HSP83, MPI, and GP63 gene expression levels were statistically upregulated in LRV2 + isolates compared to LRV2- isolates (P < 0.05). Although HSP70 and CP genes showed slight up-regulation in LRV2 + isolates, it was not statistically significant compared to LRV2- isolates.
The notable increase in gene expression levels, particularly for GP63, HSP83, and MPI genes, suggests that the presence of LRV2 + may significantly influence the expression of these factors in L. major clinical isolates.
Not applicable.
利什曼病是一种由细胞内原生动物利什曼原虫引起的寄生虫病。包括宿主免疫力和利什曼原虫种类在内的多种因素会影响该病的表现和严重程度。最近的研究揭示了利什曼原虫RNA病毒（LRV）在利什曼病临床预后中可能具有的重要作用。本研究旨在探讨LRV2 +对利什曼原虫各种致病基因的影响。
在本研究中，从诊断为皮肤利什曼病（CL）的患者中获得了35株利什曼原虫分离株。分别采用PCR-RFLP和半巢式PCR方法鉴定利什曼原虫种类和LRV2 +的存在情况。此外，使用RT-qPCR评估LRV2 +和LRV2 -利什曼原虫临床分离株中半胱氨酸蛋白酶（CP）、热休克蛋白70（HSP70）、热休克蛋白83（HSP83）、糖蛋白63（GP63）和甘露糖磷酸异构酶（MPI）的RNA表达水平。
在35株分离株中，从CL患者中选取了20株，均确诊为硕大利什曼原虫。这些分离株分为两组，LRV2 +组和LRV2 -组，每组10株。RT-qPCR分析显示，与LRV2 -分离株相比，LRV2 +分离株中HSP83、MPI和GP63基因表达水平在统计学上上调（P < 0.05）。虽然HSP70和CP基因在LRV2 +分离株中显示出轻微上调，但与LRV2 -分离株相比无统计学意义。
基因表达水平的显著增加，特别是GP63、HSP83和MPI基因，表明LRV2 +的存在可能显著影响硕大利什曼原虫临床分离株中这些因子的表达。
不适用。
