Ng K H, Lanéelle M A
Biochimie. 1977;59(1):97-104. doi: 10.1016/s0300-9084(77)80092-8.
An analysis of the free lipids of Hansenula anomala was performed. The main fatty acids obtained by saponification of whole cell crude lipids were palmitic, C18:1, C18:2 and C18:3 acids. In mitochondrial lipids the tri-unsaturated acid was present as traces. Fatty acid composition of each class of lipids was also determined. Phosphatidylcholine and phosphatidylethanolamine were the main phospholipids; phosphatidylserine, phosphatidylinositol and cardiolipin were also characterized. The most abundant sterols were ergosterol and lanosterol. An acetate of a 24-ethyl cholesterol was also isolated. Two glycolipids, a galactosyl diglyceride and a glucosyl ceramide were identified; concerning the galactosyl diglyceride, the content of C18:3 acid was higher than in other lipid classes. In the glucosyl ceramide, the main fatty acid was alpha-hydroxy C18:0 acid; C16:0, C18:1, C18:2 and C18:3 acids were present too. The long chain base was shown to be C18-phytosphingosine (4-hydroxy C18 sphinganine). Some similarities and differences with Saccharomyces cerevisiae are discussed.
对异常汉逊酵母的游离脂质进行了分析。通过对全细胞粗脂质进行皂化得到的主要脂肪酸为棕榈酸、C18:1、C18:2和C18:3酸。在线粒体脂质中，三不饱和酸以痕量存在。还测定了各类脂质的脂肪酸组成。磷脂酰胆碱和磷脂酰乙醇胺是主要的磷脂；磷脂酰丝氨酸、磷脂酰肌醇和心磷脂也得到了表征。最丰富的甾醇是麦角甾醇和羊毛甾醇。还分离出了一种24-乙基胆固醇的乙酸酯。鉴定出两种糖脂，一种半乳糖基甘油二酯和一种葡糖神经酰胺；关于半乳糖基甘油二酯，C18:3酸的含量高于其他脂质类别。在葡糖神经酰胺中，主要脂肪酸是α-羟基C18:0酸；C16:0、C18:1、C18:2和C18:3酸也存在。长链碱显示为C18-植物鞘氨醇（4-羟基C18鞘氨醇）。讨论了与酿酒酵母的一些异同。
