Li Meng, Hu Xiantong, Liu Xiaofei, Zhao Lingzhou, Zhao Wanmin, Li Ying, Bie Xiaomei, Jiao Hua, Zhao Yantao, Ma Chufan
Air Force Clinical College, The Fifth School of Clinical Medicine, Anhui Medical University, Hefei, 230032, China.
Department of Stomatology, Air Force Medical Center, Beijing, 100142, China.
Mater Today Bio. 2025 Feb 21;31:101604. doi: 10.1016/j.mtbio.2025.101604. eCollection 2025 Apr.
Bone defects resulting from trauma, tumor resection, non-union of fractures, and infections present enormous challenges in treatment. Although three-dimensional (3D) bioprinting plays an important role in repairing bone tissues, the lack of mechanical properties and osteoinductive ability of the bioinks remains a barrier for the application of the technology. In this study, we used advanced 3D bioprinting technology to create a novel piezoelectric hydrogel scaffold (Gel/PBT@BMSCs) which consisted of bone marrow-derived mesenchymal stem cells (BMSCs), gelatin methacryloyl (GelMA), and polyethylene glycol (PEG)-modified barium titanate (BT) nanoparticles. The piezoelectric hydrogel scaffold provided a stable 3D microenvironment for cell growth and adhesion, enhancing cell viability and osteogenic activity when subjected to low-intensity pulsed ultrasound (LIPUS) stimulation. Furthermore, experiments demonstrated that the innovative hydrogel scaffold significantly accelerated the healing process of bone defects and exhibited impressive bone regeneration capabilities. These findings highlight the potential of piezoelectric hydrogel for further research and application in the field of bone tissue engineering, and offer new approaches for the treatment of bone defects.
由创伤、肿瘤切除、骨折不愈合和感染导致的骨缺损在治疗上面临着巨大挑战。尽管三维（3D）生物打印在骨组织修复中发挥着重要作用，但生物墨水缺乏机械性能和骨诱导能力仍然是该技术应用的障碍。在本研究中，我们使用先进的3D生物打印技术创建了一种新型压电水凝胶支架（Gel/PBT@BMSCs），它由骨髓间充质干细胞（BMSCs）、甲基丙烯酰化明胶（GelMA）和聚乙二醇（PEG）修饰的钛酸钡（BT）纳米颗粒组成。该压电水凝胶支架为细胞生长和黏附提供了稳定的3D微环境，在受到低强度脉冲超声（LIPUS）刺激时可提高细胞活力和成骨活性。此外，实验表明，这种创新的水凝胶支架显著加速了骨缺损的愈合过程，并展现出令人印象深刻的骨再生能力。这些发现凸显了压电水凝胶在骨组织工程领域进一步研究和应用的潜力，并为骨缺损的治疗提供了新方法。
