Peltek O, Kopoleva E A, Zyuzin M V
Junior Researcher, Physics Department; ITMO University, 49, Bldg. A, Kronverksky Pr., Saint Petersburg, 197101, Russia.
Engineer, Physics Department; ITMO University, 49, Bldg. A, Kronverksky Pr., Saint Petersburg, 197101, Russia.
Sovrem Tekhnologii Med. 2025;17(1):50-56. doi: 10.17691/stm2025.17.1.05. Epub 2025 Feb 28.
Application of fluorescent redox-sensitive nanoparticles in current biomedicine ensures high sensitivity and accuracy of biovisualization. Nanoparticles are potent as they can long circulate in the blood, where the level of glutathione is relatively low, and are destroyed in tumor cells, releasing loaded dyes or drugs. was to develop new nanoparticles based on trithiocyanuric acid for biovisualization of malignant tumors and study capabilities of the developed nanoparticles.
Nanoparticles were obtained by polycondensation of trithiocyanuric acid using iodine. Scanning and transmission electron microscopy was used for their characterization, the loading of fluorescent dyes was assessed by means of spectrophotometry. Confocal laser scanning microscopy was applied to study the impact of nanoparticles on the viability of the 4T1 and A549 cell lines as well as their interaction with cells. The distribution of nanoparticles in tissues and organs of BALB/c model mice with grafted tumors was performed using fluorescence visualization.
According to scanning microscopy, the size of the synthesized particles reached 100±20 nm. The adsorption isotherm demonstrated that adsorption of 0.27 mg of the RhB fluorescent dye per 1 mg of nanoparticles could be achieved. Enhanced release of the packed fluorescent dye was seen in the presence of glutathione and acetylcysteine. The particles did not significantly affect the viability of 4T1 and A549 cells. After intratumoral administration, they ensured a more intense fluorescent signal in the tumor area compared to a regular fluorescent dye solution.
The developed system of trithiocyanuric-acid-based nanoparticles demonstrated high efficiency in biovisualization of malignant tumors and has a potential for targeted delivery of treatment agents.
荧光氧化还原敏感纳米颗粒在当前生物医学中的应用确保了生物可视化的高灵敏度和准确性。纳米颗粒具有强大的功能，因为它们可以在血液中长时间循环，而血液中谷胱甘肽水平相对较低，并且在肿瘤细胞中被破坏，释放出负载的染料或药物。本研究旨在开发基于三聚硫氰酸的新型纳米颗粒用于恶性肿瘤的生物可视化，并研究所开发纳米颗粒的性能。
通过三聚硫氰酸与碘的缩聚反应制备纳米颗粒。使用扫描电子显微镜和透射电子显微镜对其进行表征，通过分光光度法评估荧光染料的负载情况。应用共聚焦激光扫描显微镜研究纳米颗粒对4T1和A549细胞系活力的影响以及它们与细胞的相互作用。使用荧光可视化技术观察纳米颗粒在移植肿瘤的BALB/c模型小鼠组织和器官中的分布。
根据扫描显微镜观察，合成颗粒的尺寸达到100±20 nm。吸附等温线表明，每1 mg纳米颗粒可吸附0.27 mg罗丹明B荧光染料。在谷胱甘肽和乙酰半胱氨酸存在的情况下，观察到包裹的荧光染料释放增强。这些颗粒对4T1和A549细胞的活力没有显著影响。瘤内给药后，与常规荧光染料溶液相比，它们在肿瘤区域确保了更强的荧光信号。
所开发的基于三聚硫氰酸的纳米颗粒系统在恶性肿瘤的生物可视化方面显示出高效率，并且具有靶向递送治疗剂的潜力。
